IADR Abstract Archives
The Effect of Dentin Location on Erosion Depth on Acid-Challenge
Objectives: To evaluate the effect of dentin location on the erosion depth as measured with polarized light microscopy.
Methods: A total of twenty-four extracted sound human molars were obtained and randomly divided into four groups (n=6/per group), including Group A: Occlusal Superficial Dentin; Group B: Occlusal Deep Dentin; Group C: Longitudinal Dentin section; and Group D: mixture of superficial, deep occlusal and longitudinal dentin sections. All dentin blocks were painted with nail varnish to expose a 1x4mm unexposed surface. Groups A-C were then exposed to 1.0 citric acid (pH 3.9) for 1hr at 35°C. Group D served as the negative control and was treated with Grade 3 water for 1hr. Samples were then sectioned and erosion depth measured with polarized light microscopy. One-way ANOVA, followed by the post-hoc Tukey’s HSD test, was conducted to detect the difference in the mean drop values of surface from the unexposed surface (µm) among the four depth levels of dentin groups (alpha=0.05).
Results: One-way ANOVA revealed that there was a significant effect of depth of dentin on the drop values of surface from the unexposed surface (F(3, 20)=83.28; p<0.0001). The post-hoc Tukey’s HSD test indicated that the mean drop value observed in Occlusal Deep Dentin group (mean=34.5 µm; SD=5.29µm) was significant greater than those observed in Occlusal Superficial Dentin (mean=26.34µm; SD=4.89µm) and Longitudinal Dentin (mean=24.7µm; SD=3.47µm) groups, while no significant difference was found between Occlusal Superficial Dentin and Longitudinal Dentin groups. Moreover, all those three experimental treatment groups were significantly different from the Negative Control Group, which showed a zero drop value of surface.
Conclusions: Erosion depth upon erosive challenge was influenced by the location of dentin.
Methods: A total of twenty-four extracted sound human molars were obtained and randomly divided into four groups (n=6/per group), including Group A: Occlusal Superficial Dentin; Group B: Occlusal Deep Dentin; Group C: Longitudinal Dentin section; and Group D: mixture of superficial, deep occlusal and longitudinal dentin sections. All dentin blocks were painted with nail varnish to expose a 1x4mm unexposed surface. Groups A-C were then exposed to 1.0 citric acid (pH 3.9) for 1hr at 35°C. Group D served as the negative control and was treated with Grade 3 water for 1hr. Samples were then sectioned and erosion depth measured with polarized light microscopy. One-way ANOVA, followed by the post-hoc Tukey’s HSD test, was conducted to detect the difference in the mean drop values of surface from the unexposed surface (µm) among the four depth levels of dentin groups (alpha=0.05).
Results: One-way ANOVA revealed that there was a significant effect of depth of dentin on the drop values of surface from the unexposed surface (F(3, 20)=83.28; p<0.0001). The post-hoc Tukey’s HSD test indicated that the mean drop value observed in Occlusal Deep Dentin group (mean=34.5 µm; SD=5.29µm) was significant greater than those observed in Occlusal Superficial Dentin (mean=26.34µm; SD=4.89µm) and Longitudinal Dentin (mean=24.7µm; SD=3.47µm) groups, while no significant difference was found between Occlusal Superficial Dentin and Longitudinal Dentin groups. Moreover, all those three experimental treatment groups were significantly different from the Negative Control Group, which showed a zero drop value of surface.
Conclusions: Erosion depth upon erosive challenge was influenced by the location of dentin.