Signaling molecules involved in initial formation of blood vessels during tooth development

Objectives: Angiogenesis is an important process involved in growth and development but detailed mechanism of formation of blood vessels in tooth germ remains unclear. The purpose of this study was to investigate signaling molecules involved in initial formation of blood vessels during tooth development.
Methods: We performed in situ hybridization analysis using the probes of the angiogenesis-related molecules and analyzed the expression patterns of them. At the same time, we stained serial sections of mouse whole embryos immunohistochemically with the antibody against them.
Results: Localizations of the angiogenesis-related molecules were observed in dental papilla and mesenchymal layers. And also the stage-specific expression patterns were observed around the blood vessels during tooth development.
Conclusions: In conclusion, we confirmed the signaling molecules might have involved in initial formation of blood vessels during tooth development.
*This work was supported by JSPS KAKENHI Grant Numbers 22592052, 26462800.