IADR Abstract Archives
Tufts and Spindles in the Dentine-Enamel Junction. Raman spectroscopy analysis
Objectives: Objectives of this study were to investigate the chemical composition of the overall dentine-enamel
junction bristly with tufts and spindle by confocal Raman microscopy.
Methods: Raman Data Acquisition : Extracted teeth were sectioned in the longitudinal axis by means of an Isomet
diamond saw with a thickness of 0.25 mm, polished using diamond pastes (6 μm, 1 μm and 0.25 μm). Finally, the
sample was passed through an ultrasonic bath for 5 minutes.Raman spectra were collected using a Witec Confocal
Raman Microscope System alpha 300R (Witec Inc., Ulm, Germany). Data acquisition and treatment was performed
using Image Plus software from Witec.
Results: k-mean cluster analysis allow to identified an organic rich layer (10μm width) close to the dentine-enamel
junction area. Mineral/Organic, Amide I/III versus Carbonate ratios decreased in this specific area too. Spindles in
relation with dentine tubuli and tufts showed reduced mineral composition and displays spectra similar to the dentine
albeit in the enamel. Presence of lipids were also described.
Conclusions: This study confirmed the interest of using confocal Raman microscopy to study tooth structures with a
high special resolution and a high chemical sensitivity.
junction bristly with tufts and spindle by confocal Raman microscopy.
Methods: Raman Data Acquisition : Extracted teeth were sectioned in the longitudinal axis by means of an Isomet
diamond saw with a thickness of 0.25 mm, polished using diamond pastes (6 μm, 1 μm and 0.25 μm). Finally, the
sample was passed through an ultrasonic bath for 5 minutes.Raman spectra were collected using a Witec Confocal
Raman Microscope System alpha 300R (Witec Inc., Ulm, Germany). Data acquisition and treatment was performed
using Image Plus software from Witec.
Results: k-mean cluster analysis allow to identified an organic rich layer (10μm width) close to the dentine-enamel
junction area. Mineral/Organic, Amide I/III versus Carbonate ratios decreased in this specific area too. Spindles in
relation with dentine tubuli and tufts showed reduced mineral composition and displays spectra similar to the dentine
albeit in the enamel. Presence of lipids were also described.
Conclusions: This study confirmed the interest of using confocal Raman microscopy to study tooth structures with a
high special resolution and a high chemical sensitivity.