Predicting the chemo-sensitivity for cisplatin in oral squamous cell carcinoma

Objectives: Our aim is to predict chemo-sensitivity of oral squamous cell carcinoma before chemotherapy in this study. We sometimes face the pathological finding including ineffective chemotherapy or magnificent effect which could avoid surgical operation. [M1] The prediction provide major benefits including avoiding useless chemotherapy or surgical intervention. Recently, by using single cloning method, we had identified several new genes those are anti-cisplatin resistance

[M1]What you want to say, its not clear to me.


Methods: We established several cell lines, then the chemo-sensitivity and the gene expression profiles of those cell lines were investigated by cell survival assay and microarray analysis respectively. We estimated and identified intrinsic genes which expression level were correlated with the degree of resistance.
Results: Several genes including Osteopontin were identified as resistance genes to cisplatin. Osteopontin was shown to be related with response of cisplatin by using overexpression technique in vitro due to increase of AKT activation. Furthermore, RT-PCR results revealed that mRNA expression of Osteopontin in tumor tissue was correlated with cisplatin resistance.
Conclusions: These results suggested that our method is suitable to identify resistance of some genes to cisplatin and these genes may be useful for predicting the response against anti-cancer drugs.