To assess:
Method:
HA sintered at 800C for 11 hours and beta TCP sintered at 975 C for 11 hours in were used in the ratio of 15:85 as ink mixture for 3D Robocad printing. The scaffolds were resintered at 1100C for 4 hours .
Samples were HA & beta TCP powders, HA & beta TCP powders calcined at 800 C and 975C respectively for 11 hours, Unsintered HA-beta TCP scaffolds, Sintered HA-beta TCP scaffolds, HA-beta TCP scaffolds with dipyridamole in Calcium sulfate slurry and stored at 4C analyzed on Day 1 and Day 15, HA-beta TCP scaffolds with dipyridamole in calcium sulfate slurry and then dipped in dipyridamole stored at 4C in a 96 well plate and analyzed on Day 1 and Day 15, HA- beta TCP scaffolds dipped in dipyriidamole and stored at 4C and analyzed on Day 1 and Day 15, HA-beta TCP with dipyridamole stored at 37C and analyzed on Day 1 and Day 15. SEM, Micro CT, ICP and XRD were done on these samples
Result: Characterization showed macro and micro porosity incorporated into the scaffold geometric design that assisted the cells and the newly growing tissue to grow into the scaffold thus allowing bone growth, osteoconduction, vascularisation, and bone marrow formation. It revealed difference in structure post sintering
Conclusion: The HA- beta TCP scaffold provide with the physical and chamical characteristics that are needed in a scaffold for bone regeration and is biocompatible