Objective: The aim of the present in vitro study was to investigate the influence of Myrecetin treatment on the adherence of Candida albicanson oral cells.
Method: The natural occurring flavonoid myricetin (purity level >98%) was used in this study. The adherence of C. albicans (ATCC: 90028) to oral fibroblast (ATCC: CRL-7421) monolayers was determined with the addition of Myrecetin at sub-candicidal or minimum inhibitory concentrations (MIC). All tests were compared to controls. Cells were incubated with Candida in the presence of the drug in RPMI / 10% FBS for 24 h at 37°C, 5% CO2 (n=9). The toxicity in oral fibroblasts was evaluated using fluorometric quantification of cellular viability. In addition, the morphological characteristics of the cells were observed in microscope.
Result: C. albicans adherence to mammalians cells were significantly reduced (p<0.05) when Myrecetin was present, reducing adherence by 90% and 60%, at the MICs and sub-candicidal concentrations, respectively. The fibroblast viability was not affected at MIC or sub-candicidal doses.
Conclusion: The data show that sub-candicidal levels of myrecetin modulate C. albicans adherence and its colonization and have a potential to prevent candida related oral diseases.