Method:The nanofibrous gelatin/Magnesium scaffolds were prepared by combining a sol-gel process with a thermally induced phase separation method. The composition of the hybrid scaffolds were measured with Fourier transforms infrared spectroscopy (FTIR) and energy dispersive X-ray spectroscopy (EDS). The release of Mg2+ was measured by Inductive Coupled Plasma Emission Spectrometer (ICP). hDPSCs were seeded onto the scaffolds and cultured under different media for odontogenic differentiation. For in vitro study, DNA assay, alkaline phosphatase activity, real time PCR, hematoxylin and eosin (H&E) and von Kossa staining were examined. For in vivo study, X-ray, hematoxylin and eosin (H&E) and immunohistochemical staining were examined.
Result: The addition of magnesium bioactive glass (up to 10% wt %) increased the overall mechanical strength of the hybrid matrix while still retained the nanofibrous architecture of the scaffold. Magnesium was released constantly from the scaffold up to several weeks, and its release significantly enhanced the proliferation and differentiation of DPSCs in vitro. In vivo study further indicated that the addition of magnesium enhanced the dentin-pulp complex regeneration.
Conclusion:The biomimetic gelatin/magnesium hybrid scaffold is promising for dental-pulp tissue regeneration.