IADR Abstract Archives

Comparison of Human and Bovine Dentin in RDA-EP Abrasivity Assessments

Our laboratory has been developing a validated profilometry method (Radioactive Dentin Abrasion – Equivalent Profilometry RDA-EP) for the assessment of dentifrice abrasivity which is equivalent to the standard RDA method (ISO 11609 Annex A). Initial validation of the method was developed with human dentin. Expansion of the method to the use of bovine substrates could increase accessibility of the technique. Objective: This study compared bovine to human root dentin substrates in dentifrice abrasivity assessments using optimized RDA-EP method.

Methods: Three experimental dentifrices of low, medium and high abrasivity in the safe dentin abrasivity range (RDA<250) were compared using human and bovine dentin specimens (N=8/group). RDA was determined as specified in ISO 11609 Annex A. RDA-EP was determined using human and bovine dentin specimens (Vickers hardness 30-70, 0-10 microns leveling, window 3x3.5mm) with modified brushing methodology (4000 strokes, inclusion of RDA250, randomization of brushing stations, change of specimen orientation during brushing) vs. ISO11609 Annex B. Post-treatment dentin wear was analyzed by optical profilometry (Bruker Counter GT-K1) and contact profilometry (Taylor Hobson Talyform i50) using Talymap Gold 6.2 (Digitalsurf).

Results:

Treatments (RDA)	Human	Bovine
Treatments (RDA)	RDA-EP Contact /Optical Profilometry (intra-group a≠b by Fisher’s LSD p<0.05)
RDA 100 standard	100^d /100^d	100^d /100 ^cd
Low (71)	66^e / 62^e	71^d / 97^d
Med (118)	127^c / 127^c	115^c / 144^c
High (205)	166^b / 160^b	179^b / 220^b
RDA 250 (241)	250^a /250^a	250^a /250^a

Analysis of Human and Bovine dentin abrasion by bivariate fit showed RDA-EP values to be highly correlated: Optical Profilometry RMS 0.95; Stylus Profilometry RMS 0.89 (both analysis methods p<0.001).

Conclusion: Bovine substrates proved equivalent to human in comparative assessments of dentifrice abrasivity. The RDA-EP with bovine substrates would appear to be an adequate methodology for relative characterization of dentifrice abrasivity in vitro.

Division: AADR/CADR Annual Meeting
Meeting: 2014 AADR/CADR Annual Meeting (Charlotte, North Carolina)
Location: Charlotte, North Carolina
Year: 2014
Final Presentation ID: 572
Abstract Category|Abstract Category(s): Oral Health Research

Authors

Colon, Ellen ( Procter & Gamble Company, Mason, OH, USA )

Schneiderman, Eva ( Procter & Gamble Company, Mason, OH, USA )

White, Donald ( Procter & Gamble Co., Mason, OH, USA )

SESSION INFORMATION

Poster Session
Safety and Efficacy Methods for Evaluation of Oral Care Products
03/20/2014