Objective: To demonstrate the potential cross talk signaling between DPSC and MSC, to facilitate angiogenesis and differentiation, in vitro.
Methods:Human DPSC and MSC between passages 3 and 5 were cultured in osteogenic differentiation medium. At after 3 weeks, the cells were checked for its morphology. RNA isolated from the cells was used for gene expression analysis probing the differentiation markers, using real-time quantitative PCR (qRT-PCR) analysis. Migration of DPSC and MSC were monitored using Boyden trans-well membranes. Alizarin Red staining was performed to determine differentiation.
Results: DPSC (1 x 106) or MSC (1 x 106) were grown on trans-well filters and challenged with interferon-g (IFN-γ). The experimental design comprised of MSC and DPSC seeded on the bottom of a trans-well plate with the opposite lineage activated in the trans-well insert. The number of cells migrated towards each other were stained and quantified. Interestingly, our findings revealed: 1) regardless of lineage, proliferate and differentiate at a slower rate in IFN-γ challenged cells compared to control, 2) VEGF & FGF expression levels were up-regulated in DPSC exposed to IFN-γ-treated MSC, and 3) increased MSC migration (2 fold ) when compared to DPSC, exposed to IFN-γ.
Conclusion: Our studies conclude for the first time that DPSC challenged with inflammatory mediators (IFN-γ) drive the recruitment of MSC, by increasing its migration and angiogenic potential. Thus, understanding the cross-talk signaling between DPSC and MSC will bring novel insight for regenerative Endodontic therapies.