Method: The present study utilized a single centre, double blind, randomised, two-treatment, and four period crossover design. Thirty-seven subjects were enrolled and 33 completed the study. Each study period took place over a span of 15 days. Subjects were randomized to treatment sequences and received one of two marketed dentifrice products each period. Treatments included a 0.454% Stannous Fluoride dentifrice (Oral-B PRO EXPERT®) and a 0.3% Triclosan/sodium fluoride dentifrice (Colgate Total Advanced®). Subjects wore an intra-oral appliance retaining 2 polished human enamel samples for 6 hours/day, swishing with the assigned dentifrice slurry twice a day and swishing with 250ml of orange juice for 10 minutes four times/ day. Contact profilometry was used to measure surface changes of enamel samples at Baseline, days 10 and 15. Two measurements for each sample were recorded at each visit.
Result: No statistically significant treatment differences were observed at Baseline for the two dentifrice groups (p>0.44) with means of -0.015 and -0.024 for the Stannous Fluoride and the Triclosan dentifrices, respectively. At day 10 the Stannous Fluoride dentifrice demonstrated 66.8% lower enamel loss than the Triclosan dentifrice (p<0.0001) with means of 1.222µm and 3.676 µm, respectively, and at day 15, a 68.3% lower enamel loss (p<0.0001) with means of 1.595 µm and 5.027 µm, respectively. Both test products were well tolerated.
Conclusion: The in situ clinical study demonstrated the superior anti-erosion efficacy of the 0.454% Stannous Fluoride dentifrice relative to the 0.3% Triclosan/sodium fluoride dentifrice against an erosive challenge to human enamel.