Glutaraldehyde, a collagen crosslinking agent has recently gained interest for its anti-proteolytic benefits. Objectives: The objective of this study was to evaluate the effect of Gluma dentin desensitizer (5.0% glutaraldehyde and 35% HEMA, Heraeus-Kulzer) and other glutaraldehyde-containing agents on the stability of hybrid layers. Methods: Eighteen healthy molars were transversely cut to obtain dentin for bonding and a smear layer created with 600-grit SiC paper. All teeth were treated with 37% phosphoric acid (Ultradent Inc.), rinsed and blot dried. The teeth were equally and randomly assigned to six study groups with three teeth in each group and treated as follows: 1) Gluma comfort Bond (Control); 2) Gluma liquid + Gluma comfort Bond; 3) Gluma gel + Gluma comfort Bond; 4) Gluma comfort Bond & Desensitizer; 5) iBond Total Etch and 6) MicroPrime G + Gluma comfort Bond. All teeth were restored in 2mm-increments with resin composite (Filtek Z100, 3M ESPE). After 24h, all teeth were sectioned into beams of cross-sectional area of 0.9±0.1 mm2. The beams were equally divided in two groups for microtensile bond strength (μTBS) evaluation after 24h and six months of storage in artificial saliva with a universal testing machine (Bisco Inc.) at a crosshead speed of 1mm/min. Additional beams per group were immersed in a 50 wt% silver nitrate aqueous solution in the dark for 24h and then in a photo-developing solution for 8h under fluorescent light to reduce silver ions to metallic silver. The specimens were dehydrated and processed for FE-SEM evaluation. Statistical analysis of the μTBS data was done by one-way ANOVA with a significance level of p<0.05. Results: No significant differences in μTBS values were observed among treatment groups when evaluated at 24h (p>0.05). Six months results are forthcoming. Conclusion: Glutaraldehyde-containing agents do not interfere with initial bond strength to dentin.