IADR Abstract Archives
Healing of Periodontal Defects With a Novel Flowable Bone Substitute
Objectives: To evaluate the healing of intrabony defects following regenerative treatment with a novel flowable deproteinized bovine bone mineral with a collagen carrier (fDBBM-C).
Methods: Three-wall intrabony defects were created in beagle dogs and randomly assigned to one of the following three treatment modalities: Group 1: fDBBM-C, group 2: DBBM and collagen (DBBM-C), group 3: empty defects. After 4, 8, and 12 weeks respectively, the specimens were evaluated by means of micro-CT and histology.
Results: No evidence of local adverse tissue effects was observed at any timepoint. At 4 weeks, micro-CT evaluation showed that the newly formed bone and granule area values were statistically significantly higher in groups 1 (81.3±6.4%) and 2 (87.4±5.8%) compared to the negative control (64±12.3%, p<0.05). At 12 weeks the values were higher in groups 1 (79±6.4%) and 2 (82.4±12.5%) compared to group 3 (71.6±16.1%, p>0.05). Histologic analysis showed higher values for new cementum formation at 12 weeks in group 1 (90.9±42.2%) compared with group 2 (77.8±45.2%) and group 3 (84.9±29.8%), but the values did not reach statistical significance. Group 1 showed more new bone formation within the defect area compared to group 2 (54.5%±10.6% vs. 44.7±11.7%, p<0.05). The total defect fill area (new bone + biomaterial) at 12 weeks was higher in groups 1 (59.7±10.8%) and 2 (63.8±11.7%) compared to group 3 (51.2±8.4%, p<0.05). Only at 4 weeks, the test group showed a statistically significant lower bone-to-graft contact (BGC) value compared to the control group, while at 12 weeks, the BGC value was statistically significantly higher in group 1 (80.3±22.3%) compared to group 2 (62.6±14.5%, p<0.05).
Conclusions: The present results have for the first time provided histologic evidence for the potential of this novel bone substitute for periodontal and bone grafting procedures.
Methods: Three-wall intrabony defects were created in beagle dogs and randomly assigned to one of the following three treatment modalities: Group 1: fDBBM-C, group 2: DBBM and collagen (DBBM-C), group 3: empty defects. After 4, 8, and 12 weeks respectively, the specimens were evaluated by means of micro-CT and histology.
Results: No evidence of local adverse tissue effects was observed at any timepoint. At 4 weeks, micro-CT evaluation showed that the newly formed bone and granule area values were statistically significantly higher in groups 1 (81.3±6.4%) and 2 (87.4±5.8%) compared to the negative control (64±12.3%, p<0.05). At 12 weeks the values were higher in groups 1 (79±6.4%) and 2 (82.4±12.5%) compared to group 3 (71.6±16.1%, p>0.05). Histologic analysis showed higher values for new cementum formation at 12 weeks in group 1 (90.9±42.2%) compared with group 2 (77.8±45.2%) and group 3 (84.9±29.8%), but the values did not reach statistical significance. Group 1 showed more new bone formation within the defect area compared to group 2 (54.5%±10.6% vs. 44.7±11.7%, p<0.05). The total defect fill area (new bone + biomaterial) at 12 weeks was higher in groups 1 (59.7±10.8%) and 2 (63.8±11.7%) compared to group 3 (51.2±8.4%, p<0.05). Only at 4 weeks, the test group showed a statistically significant lower bone-to-graft contact (BGC) value compared to the control group, while at 12 weeks, the BGC value was statistically significantly higher in group 1 (80.3±22.3%) compared to group 2 (62.6±14.5%, p<0.05).
Conclusions: The present results have for the first time provided histologic evidence for the potential of this novel bone substitute for periodontal and bone grafting procedures.