IADR Abstract Archives
Coaggregation With Actinomyces Naeslundii Induces Cell Wall Biosynthesis, Septation and Cell Lengthening in Corynebacterium Matruchotii
Objectives: Corynebacterium matruchotii is a geometrically eccentric filamentous organism and a major constituent of supragingival dental plaque that projects hundreds of microns outward from the tooth surface. We hypothesized that C. matruchotii coaggregates with Actinomyces species and and this binding induces cooperative biophysical and biochemical interactions.
Methods: We employed live cell imaging, fluorescence in situ hybridization and dualRNAseq to investigate C. matruchotii interactions with A. naeslundii.
Results: When coaggregated with A. naeslundii, C. matruchotii filaments grow longer, they extend 2-3 fold further away from coaggregation nucleation centroids, and filaments become highly septate. RNAseq identified a total of 192 differentially expressed genes when C. matruchotii coaggregates with A. naeslundii. Conspicuously up-regulated C. matruchotii genes included components of the Type VII secretion system and genes involved in cell wall biosynthesis, septa formation, and cell division. mRNA FISH labeling identified many up-regulated genes to be segmented in compartments in long C. matruchotii filaments in proximity to A. naeslundii binding.
Conclusions: Together, these results suggest that biophysical coaggregation with A. naeslundii induces a pro-growth phenotype in C. matruchotii that results in the long filamentous cell morphology characteristic of this species in its dental plaque habitat.
Methods: We employed live cell imaging, fluorescence in situ hybridization and dualRNAseq to investigate C. matruchotii interactions with A. naeslundii.
Results: When coaggregated with A. naeslundii, C. matruchotii filaments grow longer, they extend 2-3 fold further away from coaggregation nucleation centroids, and filaments become highly septate. RNAseq identified a total of 192 differentially expressed genes when C. matruchotii coaggregates with A. naeslundii. Conspicuously up-regulated C. matruchotii genes included components of the Type VII secretion system and genes involved in cell wall biosynthesis, septa formation, and cell division. mRNA FISH labeling identified many up-regulated genes to be segmented in compartments in long C. matruchotii filaments in proximity to A. naeslundii binding.
Conclusions: Together, these results suggest that biophysical coaggregation with A. naeslundii induces a pro-growth phenotype in C. matruchotii that results in the long filamentous cell morphology characteristic of this species in its dental plaque habitat.