IADR Abstract Archives
Innovative Polymerizable Vaterite-Based Dentin Desensitizer
Objectives: 1. To formulate a dentin desensitizer utilizing vaterite, the most unstable phase of calcium carbonate, combined with bis[2-(methacryloyloxy) ethyl] phosphate (BMEP), a polymerizable self-etching monomer.
2. To characterize the interaction of the experimental desensitizer with simulated body fluid (SBF) at 1, 3, 7, 14, and 30 days.
3. To assess the effectiveness of the experimental desensitizer in occluding dentinal tubules.
Methods: The experimental desensitizer was formulated by combining BMEP, 2-hydroxyethyl methacrylate (HEMA), ethanol water, vaterite, and photoinitiators. The interaction of the photopolymerized desensitizer with SBF was analyzed using Fourier-transform infrared spectroscopy (FTIR) after incubation at the specified time points. Dentin discs (n=3/group) with exposed tubules were treated with the experimental desensitizer using a microbrush and then light-cured with a dental curing unit. A fluoride varnish served as the control, applied according to the manufacturer’s instructions. The occlusion of the dentinal tubules was assessed using scanning electron microscopy (SEM), and the percentage of tubule occlusion was calculated after 24 hours and 2 weeks.
Results: The experimental desensitizer exhibited a homogeneous paste that demonstrated curability. FTIR analysis revealed that the interaction of the desensitizer with SBF maintained the presence of vaterite at 745 cm-1 (ν4(CO3)) over 30 days, while phosphate peaks intensified with longer incubation. New peaks were observed at 874 cm-1 (ν2(CO3)), 1564 cm-1 (ν3(CO3)), and 1652 cm-1 (-OH), indicating a phase transformation. SEM images illustrated the formation of plate-like particles within the tubules following the application of the experimental desensitizer, suggesting that vaterite, in conjunction with BMEP, effectively infiltrated the tubules and interacted with SBF. The percentage of tubule occlusion supported these findings.
Conclusions: The experimental formulation desensitizer functioned as a carrier for vaterite, which localized within the dentinal tubules. This could prove beneficial for further remineralization and effective management of dentin hypersensitivity.
2. To characterize the interaction of the experimental desensitizer with simulated body fluid (SBF) at 1, 3, 7, 14, and 30 days.
3. To assess the effectiveness of the experimental desensitizer in occluding dentinal tubules.
Methods: The experimental desensitizer was formulated by combining BMEP, 2-hydroxyethyl methacrylate (HEMA), ethanol water, vaterite, and photoinitiators. The interaction of the photopolymerized desensitizer with SBF was analyzed using Fourier-transform infrared spectroscopy (FTIR) after incubation at the specified time points. Dentin discs (n=3/group) with exposed tubules were treated with the experimental desensitizer using a microbrush and then light-cured with a dental curing unit. A fluoride varnish served as the control, applied according to the manufacturer’s instructions. The occlusion of the dentinal tubules was assessed using scanning electron microscopy (SEM), and the percentage of tubule occlusion was calculated after 24 hours and 2 weeks.
Results: The experimental desensitizer exhibited a homogeneous paste that demonstrated curability. FTIR analysis revealed that the interaction of the desensitizer with SBF maintained the presence of vaterite at 745 cm-1 (ν4(CO3)) over 30 days, while phosphate peaks intensified with longer incubation. New peaks were observed at 874 cm-1 (ν2(CO3)), 1564 cm-1 (ν3(CO3)), and 1652 cm-1 (-OH), indicating a phase transformation. SEM images illustrated the formation of plate-like particles within the tubules following the application of the experimental desensitizer, suggesting that vaterite, in conjunction with BMEP, effectively infiltrated the tubules and interacted with SBF. The percentage of tubule occlusion supported these findings.
Conclusions: The experimental formulation desensitizer functioned as a carrier for vaterite, which localized within the dentinal tubules. This could prove beneficial for further remineralization and effective management of dentin hypersensitivity.
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