IADR Abstract Archives
Mechanochemically Reprogrammed Tantalum Coatings With Osteogenic and Anti-Inflammatory Properties
Objectives: Implant loosening is a common complication associated with dental implants, characterized by excessive inflammatory responses and loss of osseointegration. This study aims to develop a novel tantalum implant surface coating and evaluate the effectiveness of its "brick wall-like" surface structure and epigallocatechin gallate (EGCG)-loaded coating in modulating inflammatory responses and promoting osteogenesis through physicochemical pathways.
Methods: A tantalum surface with a brick wall-like structure was created via anodization, and EGCG was loaded onto this surface through in-situ deposition at a series of concentrations (0, 20, 40, 60 μg/ml). The biocompatibility of the coating with BMSCs osteoblasts and RAW264.7 macrophages was then assessed using CCK-8 and live/dead staining. The anti-inflammatory behavior of the samples was evaluated through flow cytometry, immunofluorescence staining, and real-time quantitative polymerase chain reaction(qPCR). Subsequently, the impact on osteogenic differentiation was assessed through alkaline phosphatase (ALP) and alizarin red staining(ARS). Statistical analyses were conducted using one-way analysis of variance (ANOVA) and Tukey's test (α = 0.05).
Results: None of the tested samples exhibited significant cytotoxicity, the 40 μg/ml group showed the best cell viability under inflammatory conditions. Flow cytometry and immunofluorescence staining revealed a notable decrease in the proportion of inflammatory macrophages on both EGCG-loaded and EGCG-unloaded brick wall-like structured coatings when compared to the untreated tantalum surface. Additionally, qPCR results showed the expression levels of inflammation-related genes, including iNOS, IL-6, and TNF-α, were significantly decreased (p<0.05). Moreover, the coating enhanced the ALP activity of osteoblasts and calcium salt deposition, which was confirmed by ALP and ARS.
Conclusions: This study demonstrated that the developed tantalum implant surface coating had good biocompatibility and could downregulate inflammatory responses and promoted osteogenesis through physicochemical pathways. It shows the clinical potential to reduce the risk of implant loosening complications as a dental implant.
Methods: A tantalum surface with a brick wall-like structure was created via anodization, and EGCG was loaded onto this surface through in-situ deposition at a series of concentrations (0, 20, 40, 60 μg/ml). The biocompatibility of the coating with BMSCs osteoblasts and RAW264.7 macrophages was then assessed using CCK-8 and live/dead staining. The anti-inflammatory behavior of the samples was evaluated through flow cytometry, immunofluorescence staining, and real-time quantitative polymerase chain reaction(qPCR). Subsequently, the impact on osteogenic differentiation was assessed through alkaline phosphatase (ALP) and alizarin red staining(ARS). Statistical analyses were conducted using one-way analysis of variance (ANOVA) and Tukey's test (α = 0.05).
Results: None of the tested samples exhibited significant cytotoxicity, the 40 μg/ml group showed the best cell viability under inflammatory conditions. Flow cytometry and immunofluorescence staining revealed a notable decrease in the proportion of inflammatory macrophages on both EGCG-loaded and EGCG-unloaded brick wall-like structured coatings when compared to the untreated tantalum surface. Additionally, qPCR results showed the expression levels of inflammation-related genes, including iNOS, IL-6, and TNF-α, were significantly decreased (p<0.05). Moreover, the coating enhanced the ALP activity of osteoblasts and calcium salt deposition, which was confirmed by ALP and ARS.
Conclusions: This study demonstrated that the developed tantalum implant surface coating had good biocompatibility and could downregulate inflammatory responses and promoted osteogenesis through physicochemical pathways. It shows the clinical potential to reduce the risk of implant loosening complications as a dental implant.