IADR Abstract Archives
Systemic Fluoxetine Alleviates Inflammation and Bone Loss in Stressed Rats
Objectives: To evaluate the effect of systemic fluoxetine administration (a selective serotonin reuptake inhibitor) on inflammatory and resorptive mechanisms in apical periodontitis (AP) under chronic stress.
Methods: Twenty-four 3-month-old Wistar rats were assigned to three groups (n=8): a non-stressed AP group (AP), a stressed AP group (AP+S), and a stressed AP group treated with fluoxetine (AP+S+FLX). The AP+S and AP+S+FLX groups underwent six weeks of unpredictable chronic stress, with daily administration of saline or fluoxetine via gavage. AP was induced on day 21, and all animals were euthanized at the end of the sixth week. Serum ACTH and corticosterone levels were measured (Multiplex assay), while histological analysis, RT-PCR (RANKL, OPG, TRAP, IL-6, IL-10, IL-17), and micro-CT assessed inflammation and bone resorption. Statistical analyses included ANOVA with Tukey’s test for parametric data and the Kruskal-Wallis test with Dunn’s post hoc for non-parametric data (p<0.05).
Results: Serum ACTH levels were significantly higher in the AP+S group compared to the AP group (P=0.021). No significant differences were observed in corticosterone levels (P>0.05). The AP+S group showed a significantly higher median inflammation score (4) than the AP group (2) (P=0.04), while the AP+S+FLX group (2.5) showed no significant differences from the other groups (P>0.05). Gene expression of OPG was significantly higher in the AP+S+FLX group compared to the AP+S group (P=0.0054 and P=0.0033, respectively). Conversely, IL-6 and IL-17 gene expression levels were significantly lower in the AP+S+FLX group compared to the AP+S group (P<0.0001 and P=0.0228, respectively). No significant differences were observed in RANKL, TRAP, or IL-10 gene expression (P>0.05). The AP+S+FLX group exhibited reduced bone resorption compared to the AP+S group (P=0.02).
Conclusions: Systemic fluoxetine reduced inflammation and bone resorption linked to AP under chronic stress, suggesting a potential modulatory role in its inflammatory and resorptive mechanisms.
Methods: Twenty-four 3-month-old Wistar rats were assigned to three groups (n=8): a non-stressed AP group (AP), a stressed AP group (AP+S), and a stressed AP group treated with fluoxetine (AP+S+FLX). The AP+S and AP+S+FLX groups underwent six weeks of unpredictable chronic stress, with daily administration of saline or fluoxetine via gavage. AP was induced on day 21, and all animals were euthanized at the end of the sixth week. Serum ACTH and corticosterone levels were measured (Multiplex assay), while histological analysis, RT-PCR (RANKL, OPG, TRAP, IL-6, IL-10, IL-17), and micro-CT assessed inflammation and bone resorption. Statistical analyses included ANOVA with Tukey’s test for parametric data and the Kruskal-Wallis test with Dunn’s post hoc for non-parametric data (p<0.05).
Results: Serum ACTH levels were significantly higher in the AP+S group compared to the AP group (P=0.021). No significant differences were observed in corticosterone levels (P>0.05). The AP+S group showed a significantly higher median inflammation score (4) than the AP group (2) (P=0.04), while the AP+S+FLX group (2.5) showed no significant differences from the other groups (P>0.05). Gene expression of OPG was significantly higher in the AP+S+FLX group compared to the AP+S group (P=0.0054 and P=0.0033, respectively). Conversely, IL-6 and IL-17 gene expression levels were significantly lower in the AP+S+FLX group compared to the AP+S group (P<0.0001 and P=0.0228, respectively). No significant differences were observed in RANKL, TRAP, or IL-10 gene expression (P>0.05). The AP+S+FLX group exhibited reduced bone resorption compared to the AP+S group (P=0.02).
Conclusions: Systemic fluoxetine reduced inflammation and bone resorption linked to AP under chronic stress, suggesting a potential modulatory role in its inflammatory and resorptive mechanisms.