Probiotic LAB813 Regulates Oral Cancer Cell Behaviour

Objectives: i) To characterize the cellular and molecular changes in UMSCC-1 cells.
ii) To test the effects of LAB813 on cell migration and invasion.
Methods: UMSCC-1 was exposed to LAB813 at multiplicities of infection (MOI) of 1:250 and 1:500 for 2 and 5 h. Changes in UMSCC-1 morphology were subsequently analyzed by microscopy. Gene expression (IL-6, IL-8, IL-1α, MMP-9, CXCL4) was quantified by RT-qPCR. Cytokine release (IL-6, IL-8) was measured by ELISA assays. Scratch and invasion assays were performed to evaluate the cell migration of UMSCC-1 cells after exposure to LAB813.
Results: UMSCC-1 cells, upon exposure to LAB813, exhibited tighter intercellular adhesion and presented with extensive filopodia-like extensions. A 5-h-exposure of UMSCC-1 to LAB813 at a MOI of 500 showed decreasing IL-6, IL-8, IL-1α, and MMP-9 gene expression. In contrast, the tumor suppression CXCL4 gene was found increased. Scratch and invasion assays showed a reduced ability of UMSCC-1 cells to migrate and invade upon exposure to LAB813.
Conclusions: Our data strongly suggest a beneficial role for LAB813 in modulating the inflammatory and invasive activities of UMSCC-1. Because of its anticancer activities, LAB813 probiotic has the potential to be developed as a novel and natural therapeutic for cancer prevention.