IADR Abstract Archives
S100A7 Expression in Margins of Resected Oral Squamous Cell Carcinoma.
Objectives: To investigate the relationship between S100A7 expression in epithelium of oral squamous cell carcinoma (OSCC) resection margins and local recurrence.
Methods: Twenty maxillectomy cases of OSCC and available pre-resection and/or post-resection biopsies were reviewed. Formalin fixed, paraffin embedded tissue blocks were stained for S100A7 protein using immunohistochemical methods. Proportion (0-5) and intensity (0-3) scores were assigned generating an overall staining score (OSS) ranging from 0-8.
On whole images of stained slides, QuPath software was used to calculate the ratio of stained area over total area within an epithelial region of interest. An S100A7 immunohistochemistry-based signature algorithm (S100A7ARS) was used to determine a risk score and probability of OSCC recurrence, as low (0-21%), medium (22-54%) and high risk (55+%).
Results: Thirteen patients were male and seven were female, all over the age of 55 years. 36% were smokers and 71% drank alcohol. OSCC recurred in 6 cases. Logistic regression showed that higher risk rating by S100A7ARS and OSS were significantly associated with recurrence.
Pearson’s correlation showed a significant relationship between OSS and tumor recurrence and for S100A7ARS probability and tumor recurrence, but not for QuPath analysis. S100A7ARS probability had a significant correlation with OSS and QuPath analysis.
Significant differences occurred in the S100A7ARS probability between pre-resection biopsy and resected tissue; in S100A7ARS between resected and post-resection biopsy tissue; in QuPath analysis between pre-resected and resected tissue; in QuPath analysis between resection and post-resection biopsy tissue; in OSS between pre-resection biopsy and resected tissue; and in OSS between resected and post-resected tissue.
Conclusions: S100A7 expression in resected OSCC margins may have utility in prediction of local recurrence of tumours. A limitation of this study is the small sample size, and increased case numbers are required to validate the result.
Methods: Twenty maxillectomy cases of OSCC and available pre-resection and/or post-resection biopsies were reviewed. Formalin fixed, paraffin embedded tissue blocks were stained for S100A7 protein using immunohistochemical methods. Proportion (0-5) and intensity (0-3) scores were assigned generating an overall staining score (OSS) ranging from 0-8.
On whole images of stained slides, QuPath software was used to calculate the ratio of stained area over total area within an epithelial region of interest. An S100A7 immunohistochemistry-based signature algorithm (S100A7ARS) was used to determine a risk score and probability of OSCC recurrence, as low (0-21%), medium (22-54%) and high risk (55+%).
Results: Thirteen patients were male and seven were female, all over the age of 55 years. 36% were smokers and 71% drank alcohol. OSCC recurred in 6 cases. Logistic regression showed that higher risk rating by S100A7ARS and OSS were significantly associated with recurrence.
Pearson’s correlation showed a significant relationship between OSS and tumor recurrence and for S100A7ARS probability and tumor recurrence, but not for QuPath analysis. S100A7ARS probability had a significant correlation with OSS and QuPath analysis.
Significant differences occurred in the S100A7ARS probability between pre-resection biopsy and resected tissue; in S100A7ARS between resected and post-resection biopsy tissue; in QuPath analysis between pre-resected and resected tissue; in QuPath analysis between resection and post-resection biopsy tissue; in OSS between pre-resection biopsy and resected tissue; and in OSS between resected and post-resected tissue.
Conclusions: S100A7 expression in resected OSCC margins may have utility in prediction of local recurrence of tumours. A limitation of this study is the small sample size, and increased case numbers are required to validate the result.
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