IADR Abstract Archives
Enhancing Vertical Bone Regeneration With Fibrin-Infused E-RhBMP-2
Objectives: Previously, we demonstrated that β-tricalcium phosphate (β-TCP) and E. coli-produced recombinant human bone morphogenetic protein-2 (E-rhBMP-2) complex is useful as a bone replacement and have started a clinical trial. However, this material is difficult to maintain its shape and lacks the mechanical strength required for vertical bone regeneration. Therefore, in this study, we investigated the possibility of improving the mechanical strength and vertical bone regeneration ability of β-TCP and E-rhBMP-2 complex by applying fibrin, a dermal adhesive material (Beriplast, CSL Behring, USA).
Methods: Three experimental groups were transplanted onto mice calvaria(n=5 per each): TCP-BMP group (β-TCP loaded with 60μg E-rhBMP-2), Fibrin-BMP/TCP-BMP group (fibrin loaded with 30μg E-rhBMP-2 and β-TCP loaded with 30μg E-rhBMP-2), and Fibrin /TCP-BMP group (fibrin without E-rhBMP-2 and β-TCP loaded with 60μg E-rhBMP-2). After 12 weeks, vertical bone regeneration was analyzed by micro-computed tomography (CT) and histological analysis. Statistical analysis was performed using one-way ANOVA and Tukey's multiple comparison.
Results: Micro-CT analysis demonstrated that the fibrin-containing groups had significantly higher initial mechanical stability and better ability to maintain vertical shape compared to the non-fibrin-containing group (p<0.05). Histological analysis revealed no significant differences in bone formation and β-TCP absorption rate between TCP-BMP group and Fibrin-BMP/TCP-BMP group. Notably, the Fibrin/TCP-BMP group showed considerably inferior results in bone formation and β-TCP resorption compared to the other groups (p<0.05). Furthermore, this group had the lowest osteoclast activity, suggesting that the absence of E-rhBMP-2 in the fibrin inhibits bone formation and β-TCP resorption.
Conclusions: These results indicate that fibrin-containing E-rhBMP-2 can enhance the mechanical strength of the β-TCP and E-rhBMP-2 complex while preserving its osteogenic potential. This approach can be a valuable solution for vertical bone regeneration, addressing the issues of maintaining shape and strength.
Methods: Three experimental groups were transplanted onto mice calvaria(n=5 per each): TCP-BMP group (β-TCP loaded with 60μg E-rhBMP-2), Fibrin-BMP/TCP-BMP group (fibrin loaded with 30μg E-rhBMP-2 and β-TCP loaded with 30μg E-rhBMP-2), and Fibrin /TCP-BMP group (fibrin without E-rhBMP-2 and β-TCP loaded with 60μg E-rhBMP-2). After 12 weeks, vertical bone regeneration was analyzed by micro-computed tomography (CT) and histological analysis. Statistical analysis was performed using one-way ANOVA and Tukey's multiple comparison.
Results: Micro-CT analysis demonstrated that the fibrin-containing groups had significantly higher initial mechanical stability and better ability to maintain vertical shape compared to the non-fibrin-containing group (p<0.05). Histological analysis revealed no significant differences in bone formation and β-TCP absorption rate between TCP-BMP group and Fibrin-BMP/TCP-BMP group. Notably, the Fibrin/TCP-BMP group showed considerably inferior results in bone formation and β-TCP resorption compared to the other groups (p<0.05). Furthermore, this group had the lowest osteoclast activity, suggesting that the absence of E-rhBMP-2 in the fibrin inhibits bone formation and β-TCP resorption.
Conclusions: These results indicate that fibrin-containing E-rhBMP-2 can enhance the mechanical strength of the β-TCP and E-rhBMP-2 complex while preserving its osteogenic potential. This approach can be a valuable solution for vertical bone regeneration, addressing the issues of maintaining shape and strength.