IADR Abstract Archives
Efficacy of Antimicrobial Wipes vs Streptococcus Mutans
Objectives: We compared various antimicrobial wipes to measure their efficacy against Streptococcus mutans.
Methods: An in vitro study was conducted with three different brands of antimicrobial wipes. Selected brands were CaviWipes HP by Metrex, Optim 1 Wipes by SciCan, and Surface Disinfectant Wipes by Darby. The methodology of this experiment was based largely on the hand sanitizer study by Jain et al in 2016. Clinical isolates of Streptococcus mutans, the largest component of the oral microbiota, were grown in Brain Heart Infusion (BHI) for twelve hours at 37°C. One mL of BHI containing S. mutans was pipetted onto blood agar plates. A plastic cell spreader was used streak the surface of the plates, three times at a 60 degree angle. Once dry, 10mm x 10mm squares of each wipe type were placed onto plates. The plates were incubated at 37°C for 48 hours, and zones of inhibition were measured and compared for the degree of susceptibility of the microorganism to the antimicrobial agent in the wipe/solution. This test was repeated three times per wipe type. ANOVA was used to test for differences in the zone of inhibition (mm) by brand of disinfecting wipe (Cavi, Optim, or Darby) (α=0.05)
Results: Results showed that one group (CaviWipes) had 0 readings for all samples indicating they failed to produce a zone of inhibition. Given the lack of group variance, CaviWipes were therefore omitted from subsequent statistical analysis. Results of the ANOVA for Darby and Optim show a significant effect of disinfectant brand on the zone of inhibition (p-value<0.05, F=9.32), with Darby producing the largest zone of antibacterial activity.
Conclusions: These results indicate that Disinfectant Wipes by Darby are highly effective at reducing S. mutans bacterial load under our test conditions.
Methods: An in vitro study was conducted with three different brands of antimicrobial wipes. Selected brands were CaviWipes HP by Metrex, Optim 1 Wipes by SciCan, and Surface Disinfectant Wipes by Darby. The methodology of this experiment was based largely on the hand sanitizer study by Jain et al in 2016. Clinical isolates of Streptococcus mutans, the largest component of the oral microbiota, were grown in Brain Heart Infusion (BHI) for twelve hours at 37°C. One mL of BHI containing S. mutans was pipetted onto blood agar plates. A plastic cell spreader was used streak the surface of the plates, three times at a 60 degree angle. Once dry, 10mm x 10mm squares of each wipe type were placed onto plates. The plates were incubated at 37°C for 48 hours, and zones of inhibition were measured and compared for the degree of susceptibility of the microorganism to the antimicrobial agent in the wipe/solution. This test was repeated three times per wipe type. ANOVA was used to test for differences in the zone of inhibition (mm) by brand of disinfecting wipe (Cavi, Optim, or Darby) (α=0.05)
Results: Results showed that one group (CaviWipes) had 0 readings for all samples indicating they failed to produce a zone of inhibition. Given the lack of group variance, CaviWipes were therefore omitted from subsequent statistical analysis. Results of the ANOVA for Darby and Optim show a significant effect of disinfectant brand on the zone of inhibition (p-value<0.05, F=9.32), with Darby producing the largest zone of antibacterial activity.
Conclusions: These results indicate that Disinfectant Wipes by Darby are highly effective at reducing S. mutans bacterial load under our test conditions.
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