IADR Abstract Archives
Lactobacilli are Enriched in ex Vivo Biofilms From Hyperglycemic Saliva
Objectives: We aimed to establish an ex vivo biofilm model using hyperglycemic saliva collected from individuals with type 2 diabetes mellitus(DM). This model was designed to investigate the oral microbiome's ability to metabolize salivary glucose. Here, we assessed the model's effectiveness in isolating Lactobacilli and Streptococcus mutans.
Methods: Volunteers were selected based on self-report diagnosis of type 2 DM while a control group without DM was also included. Levels of A1c, Decayed Missing or Filled Teeth index(DMFT), sex and age of individuals were recorded. Whole saliva was collected and analyzed for pH and glucose concentration. Aliquots were inoculated into a collagen-coated Calgary biofilm device(CBD) and incubated anaerobically (24h and 72h). Biofilms formed on CBD pegs were harvested and cultured in MRS, Mitis Salivarius and BHI agar. The number of colony forming units(CFUs) was obtained. Proportions of putative Lactobacilli and S. mutans in relation to the total microbial load were calculated and compared between biofilms formed from hyper and normoglycemic saliva.
Results: The mean A1c and DMFT were higher in DM (9.87±1.19 and 20.33±7.51) than in controls (5.15±0.07 and 8.33±8.08). Salivary glucose concentrations reduced following 24 hours of biofilm growth. The hyperglycemic groups showed slightly lower averages of pH than controls in both, initial samples and after 24h. The average proportion of Lactobacilli in the total microbial load of biofilms formed from hyperglycemic saliva (29.63%±38.53) was higher than from normoglycemic saliva (5.91%±7.47). There was a reduction in the total microbial load and proportions of S. mutans in relation to the total microbial load for DM samples.
Conclusions: In our model, the hyperglycemic saliva was enriched in Lactobacilli when compared to the normoglycemic saliva. However, the total microbial load and the proportions of S. mutans were reduced. Further steps involve the qPCR of genes responsible for the internalization of glucose in these biofilms.
Methods: Volunteers were selected based on self-report diagnosis of type 2 DM while a control group without DM was also included. Levels of A1c, Decayed Missing or Filled Teeth index(DMFT), sex and age of individuals were recorded. Whole saliva was collected and analyzed for pH and glucose concentration. Aliquots were inoculated into a collagen-coated Calgary biofilm device(CBD) and incubated anaerobically (24h and 72h). Biofilms formed on CBD pegs were harvested and cultured in MRS, Mitis Salivarius and BHI agar. The number of colony forming units(CFUs) was obtained. Proportions of putative Lactobacilli and S. mutans in relation to the total microbial load were calculated and compared between biofilms formed from hyper and normoglycemic saliva.
Results: The mean A1c and DMFT were higher in DM (9.87±1.19 and 20.33±7.51) than in controls (5.15±0.07 and 8.33±8.08). Salivary glucose concentrations reduced following 24 hours of biofilm growth. The hyperglycemic groups showed slightly lower averages of pH than controls in both, initial samples and after 24h. The average proportion of Lactobacilli in the total microbial load of biofilms formed from hyperglycemic saliva (29.63%±38.53) was higher than from normoglycemic saliva (5.91%±7.47). There was a reduction in the total microbial load and proportions of S. mutans in relation to the total microbial load for DM samples.
Conclusions: In our model, the hyperglycemic saliva was enriched in Lactobacilli when compared to the normoglycemic saliva. However, the total microbial load and the proportions of S. mutans were reduced. Further steps involve the qPCR of genes responsible for the internalization of glucose in these biofilms.