IADR Abstract Archives
Biocompatibility of a Chlorehexdine-Erythritol-Containing Adjunctive Periodontal Treatment on RMSCs
Objectives: To measure rat mesenchymal stem cell (RMSC) growth following exposure to Airflow Plus Powder (AFPP, EMS, Nyon, Switzerland), chlorhexidine gluconate (CHX; Henry Schein, Melville, NY, USA) (0.12%), and/or erythritol (ERY; Fisher Scientific, Waltham MA, USA). Both of these chemicals are contained in AFPP.
Methods: Conditioned media was prepared by combining one of the following with 10mL a-MEM: 0.1g AFPP in 200µl ethanol; 0.01g AFPP in 200µl ethanol; 200µl ethanol; 1.0g ERY (Fisher Scientific, Waltham, MA); 0.5mL CHX 0.12% (Henry Schein, Melville, NY). Media was incubated (37°C, 5%CO2; 24 hours), 0.22um-sterile filtered, and components added to make complete media. RMSCs were seeded onto 24-well plates (10,000 cells/well) with BM- media (37°C, 5%CO2). Following two days of growth, alamarBlue assay measured baseline cell numbers. Conditioned media was added to each treatment group and BM- was added to the positive control group. AlamarBlue assays were completed at 2, 8, 24hrs from application of treatment media. Separate 24-well plates were used for each time point to reduce repeated alamarBlue exposure of cells. GraphPad Prism version 9.5.1 for Windows (GraphPad Software, San Diego, California USA) was used to complete two-way repeated measures ANOVA and Šídák's multiple comparisons test (α=0.05).
Results: There was a significant effect of treatment and time on cell growth (p < 0.0001, F = 75.57). ERY and CHX caused significant decrease in cell number at each timepoint compared to baseline (Figure 1). There were decreased cell numbers in the positive control, 0.1g and 0.01g AFPP, and ETH groups at 2 and 8 hours, but cell numbers increased after 24 hours.
Conclusions: AFPP did not impact cell proliferation, indicating potential cell growth preservation in the sulcus post-periodontal treatment. ERY and CHX exhibited negative effects, but the concentration of these experimental treatments likely differed from proprietary AFPP concentrations.
Methods: Conditioned media was prepared by combining one of the following with 10mL a-MEM: 0.1g AFPP in 200µl ethanol; 0.01g AFPP in 200µl ethanol; 200µl ethanol; 1.0g ERY (Fisher Scientific, Waltham, MA); 0.5mL CHX 0.12% (Henry Schein, Melville, NY). Media was incubated (37°C, 5%CO2; 24 hours), 0.22um-sterile filtered, and components added to make complete media. RMSCs were seeded onto 24-well plates (10,000 cells/well) with BM- media (37°C, 5%CO2). Following two days of growth, alamarBlue assay measured baseline cell numbers. Conditioned media was added to each treatment group and BM- was added to the positive control group. AlamarBlue assays were completed at 2, 8, 24hrs from application of treatment media. Separate 24-well plates were used for each time point to reduce repeated alamarBlue exposure of cells. GraphPad Prism version 9.5.1 for Windows (GraphPad Software, San Diego, California USA) was used to complete two-way repeated measures ANOVA and Šídák's multiple comparisons test (α=0.05).
Results: There was a significant effect of treatment and time on cell growth (p < 0.0001, F = 75.57). ERY and CHX caused significant decrease in cell number at each timepoint compared to baseline (Figure 1). There were decreased cell numbers in the positive control, 0.1g and 0.01g AFPP, and ETH groups at 2 and 8 hours, but cell numbers increased after 24 hours.
Conclusions: AFPP did not impact cell proliferation, indicating potential cell growth preservation in the sulcus post-periodontal treatment. ERY and CHX exhibited negative effects, but the concentration of these experimental treatments likely differed from proprietary AFPP concentrations.
IMAGES
4008685_File000000.jpg
4008685_File000000.jpg