PH, Bond Strength, and Antibiofilm Activity of NeoSEALER^®Flo and NeoMTA2

Objectives: This study evaluated the physicochemical and mechanical properties, as well as the antimicrobial activity of novel bioceramic sealers.
Methods: NeoSEALER^® Flo (NSF, Avalon Biomed), a ready-to-use bioceramic root canal sealer, and NeoMTA2 (NMTA2, Avalon Biomed), a powder/gel bioceramic endodontic sealer, which may be used as a repair cement or endodontic sealer, were evaluated in comparison with AH Plus (AHP, Dentsply DeTrey). The pH (n=10) was assessed in polyethylene tubes filled with the sealers after immersion in deionized water for 1, 7, and 14 days. Additionally, bovine roots were sliced into 2mm thick sections, and the root canals were prepared with 1.5 mm diameter. After filling with tested materials (n=10), push-out bond strength was evaluated by using a universal machine. The loading speed was 0,5mm/min until the dislodgment of the material. The maximum load for the displacement of the sealers was obtained in Mpa. Furthermore, antibiofilm activity against Enterococcus faecalis was evaluated using the modified direct contact test after a 48-hour setting time (n=6), or with eluate from fresh sealers (n=6), and the Colony-forming units (CFU mL-1) were counted. Statistical analysis was conducted using ANOVA and Tukey's post hoc test (α = 0.05).
Results: NSF and NMTA2 demonstrated higher pH than AH Plus (AHP) (p < 0.05). NSF showed better bond strength, and NMTA2 was similar to AHP (p > 0.05). The antibiofilm activity of eluate sealers was higher for NSF and AHP, followed by NMTA2 (p < 0.05). When materials were evaluated after setting time, NMTA2 showed greater antibiofilm activity followed by NSF, and AHP (p < 0.05).
Conclusions: The new bioceramic sealers NeoSEALER^® Flo and NeoMTA2 demonstrate alkaline pH, proper bond strength, and antibiofilm activity in both eluate and after setting.