IADR Abstract Archives
Association of Hemoglobin With Sodium-Trimetaphosphate Reduces Initial Enamel Erosion
Objectives: This study evaluated the protective effect of human hemoglobin (Hb) associated with different concentrations of microparticulate sodium trimetaphosphate (TMP) against initial enamel erosion in vitro.
Methods: 90 bovine enamel specimens were prepared and divided into 6 treatment groups (n=15/group): 1) Deionized water (negative control); 2) Elmex ErosionTM (positive control); 3) 1.0mg/mL Hb; 4) 1.0mg/mL Hb+0.5% TMP; 5) 1.0mg/mL Hb+1% TMP and 6) 1.0mg/mL Hb+3% TMP. The specimens were individually treated with the respective solutions (200μL; 2h; 37oC), then incubated in human saliva (200μL; 2h; 37oC) for acquired pellicle formation. The specimens were submitted to erosive challenges (0.01M hydrochloric acid; pH 2.3; 1mL; 10s; 37oC). These procedures were performed once/day for 3 days. Percentage of surface hardness change (%SHC) and relative surface reflection intensity (%SRI) by the Reflectometer Optipen were submitted to statistical analysis (ANOVA and Tukey’s tests; p < 0.05).
Results: In general, the treatment with Hb and all combinations between Hb+TMP significantly protected enamel when compared to the negative and positive control groups. Regarding %SHC, the combination 1.0mg/mL Hb+0.5% TMP (11.1±3.3%) was the most effective treatment when compared to all the other treatments. In respect to %SRI the best result was found for 1.0mg/ml Hb (81.3±2.1%), which showed a significant difference when compared to Elmex ErosionTM (67.5±3.5%), deionized water (53.1±5.1%), 1.0mg/mL Hb+0.5% TMP (76.5±5.1%), 1.0mg/mL Hb+1.0% TMP (74.8±4.2) and 1.0mg/mL Hb+3.0% TMP (76.2±4.7%).
Conclusions: Hb protected enamel against initial erosion in vitro and this protective effect was potentialized by association with TMP, especially at 0.5%.
Methods: 90 bovine enamel specimens were prepared and divided into 6 treatment groups (n=15/group): 1) Deionized water (negative control); 2) Elmex ErosionTM (positive control); 3) 1.0mg/mL Hb; 4) 1.0mg/mL Hb+0.5% TMP; 5) 1.0mg/mL Hb+1% TMP and 6) 1.0mg/mL Hb+3% TMP. The specimens were individually treated with the respective solutions (200μL; 2h; 37oC), then incubated in human saliva (200μL; 2h; 37oC) for acquired pellicle formation. The specimens were submitted to erosive challenges (0.01M hydrochloric acid; pH 2.3; 1mL; 10s; 37oC). These procedures were performed once/day for 3 days. Percentage of surface hardness change (%SHC) and relative surface reflection intensity (%SRI) by the Reflectometer Optipen were submitted to statistical analysis (ANOVA and Tukey’s tests; p < 0.05).
Results: In general, the treatment with Hb and all combinations between Hb+TMP significantly protected enamel when compared to the negative and positive control groups. Regarding %SHC, the combination 1.0mg/mL Hb+0.5% TMP (11.1±3.3%) was the most effective treatment when compared to all the other treatments. In respect to %SRI the best result was found for 1.0mg/ml Hb (81.3±2.1%), which showed a significant difference when compared to Elmex ErosionTM (67.5±3.5%), deionized water (53.1±5.1%), 1.0mg/mL Hb+0.5% TMP (76.5±5.1%), 1.0mg/mL Hb+1.0% TMP (74.8±4.2) and 1.0mg/mL Hb+3.0% TMP (76.2±4.7%).
Conclusions: Hb protected enamel against initial erosion in vitro and this protective effect was potentialized by association with TMP, especially at 0.5%.