IADR Abstract Archives
Triple Functional Scaffolds Improve Bone Regeneration After Radiation Injury
Objectives: ORNJ remains a major clinical challenge. A new antibacterial, antioxidant and osteoinductive bioscaffold has been developed to promote bone regeneration for radiation-damaged bone defects.
Methods: The baicalein liposomes and cerium oxide nanoparticles were loaded into the poly(lactic-co-glycolic acid) /polycaprolactone electrospun scaffold. Silver nanoparticles were incorporated onto the scaffold surface by dopamine coating. The physicochemical properties of the scaffolds were characterized. And the antibacterial effects of the scaffolds on Staphylococcus aureus and Escherichia coli were evaluated by bacterial adhesion and inhibition zone experiments. Cell adhesion, proliferation and differentiation were evaluated using the MC3T3-E1 cell irradiation injury model. And the ability of the scaffolds to promote the repair of radiation-induced bone defects was evaluated using a rat jaw bone defect model of radiation injury.
Results: The scaffolds significantly promoted the proliferation of irradiated cells (P<0.05) and early cell differentiation (P<0.001). The scaffolds significantly upregulated the expression of BMP2, RUNX2 and OCN genes at both 7 and 14 days by PCR experiments. Cells cocultured with the bioscaffold were found to express more BMP2 and RUNX2 proteins (P<0.01) and OCN proteins (P<0.001). These results indicated that the bioscaffolds could effectively promote the differentiation of irradiation-damaged osteoblasts. The bioscaffold also significantly reduced the high ROS levels of the cells (P<0.01). The bioscaffolds were found to significantly promote new bone regeneration in the bone defect area in the rat jaw (BV/TV rate: P<0.01; BS/TV rate: P<0.01).
Conclusions: The multifunctional scaffold showed good mechanical and antibacterial properties. The in vitro experiments showed that the scaffold had good cell compatibility, effective osteogenic properties of irradiated cells and ROS scavenging properties, and the in vivo animal experiments showed that the scaffolds promoted bone regeneration in the radiation-damaged bone defect. The bioscaffold has potential applications in bone repair caused by radiation injury.
Methods: The baicalein liposomes and cerium oxide nanoparticles were loaded into the poly(lactic-co-glycolic acid) /polycaprolactone electrospun scaffold. Silver nanoparticles were incorporated onto the scaffold surface by dopamine coating. The physicochemical properties of the scaffolds were characterized. And the antibacterial effects of the scaffolds on Staphylococcus aureus and Escherichia coli were evaluated by bacterial adhesion and inhibition zone experiments. Cell adhesion, proliferation and differentiation were evaluated using the MC3T3-E1 cell irradiation injury model. And the ability of the scaffolds to promote the repair of radiation-induced bone defects was evaluated using a rat jaw bone defect model of radiation injury.
Results: The scaffolds significantly promoted the proliferation of irradiated cells (P<0.05) and early cell differentiation (P<0.001). The scaffolds significantly upregulated the expression of BMP2, RUNX2 and OCN genes at both 7 and 14 days by PCR experiments. Cells cocultured with the bioscaffold were found to express more BMP2 and RUNX2 proteins (P<0.01) and OCN proteins (P<0.001). These results indicated that the bioscaffolds could effectively promote the differentiation of irradiation-damaged osteoblasts. The bioscaffold also significantly reduced the high ROS levels of the cells (P<0.01). The bioscaffolds were found to significantly promote new bone regeneration in the bone defect area in the rat jaw (BV/TV rate: P<0.01; BS/TV rate: P<0.01).
Conclusions: The multifunctional scaffold showed good mechanical and antibacterial properties. The in vitro experiments showed that the scaffold had good cell compatibility, effective osteogenic properties of irradiated cells and ROS scavenging properties, and the in vivo animal experiments showed that the scaffolds promoted bone regeneration in the radiation-damaged bone defect. The bioscaffold has potential applications in bone repair caused by radiation injury.