Abstract Archives

IADR Abstract Archives

Interaction of the Oral Microbiome and Dietary Nitrate Intake on Cardiometabolic Benefits: Results From ORIGINS

Objectives: The enterosalivary nitrate-nitrite-nitric oxide (NO3- NO2- NO) pathway requires the oral microbiome for NO production (a signaling molecule beneficial to cardiometabolic health) following dietary nitrate intake. We hypothesize that the effects of the oral microbiome on cardiometabolic biomarker risk may be different by levels of nitrate intake.
Methods: We analyzed cross-sectional data from 657 Oral Infections Glucose Intolerance and Insulin Resistance Study (ORGINS) participants. Dietary nitrate intakes and alternative healthy eating index (AHEI) scores were calculated from food frequency questionnaire responses and a US food database. Subgingival 16S rRNA microbial genes (Illumina, MiSeq) were sequenced, and PICRUSt2 estimated metagenomic content. The Microbiome Induced Nitric oxide Enrichment Score (MINES) was calculated as a microbial gene abundance ratio representing enhanced net capacity for NO generation. Cardiometabolic biomarker (systolic and diastolic blood pressure, HbA1C, glucose, insulin, and HOMA-IR) Z-scores were averaged to form a cardiometabolic Z-score (CMZ), whereby higher CMZ indicates worse overall cardiometabolic risk. Adjusted multivariable linear models regressed CMZ and individual Z-scores on MINES, dietary nitrate intake and their interaction term, adjusting for total energy intake, sex, age, race/ethnicity, education, smoking status, body mass index, periodontal status and Alternative Healthy Eating Index.
Results: Participants were mean age 31±9, 72% female, 31% White, 14% Black, and 28% Hispanic. Mean MINES and nitrate intake were 0.57±0.3 and 189±171 mg/day. Higher MINES was significantly associated with lower CMZ. For every 1 std increase in MINES, the mean CMZ(SE) decreased by -0.35(0.12) standardized units, p =0.005. In subgroup analysis by above vs. below median nitrate intake, MINES was only associated with lower cardiometabolic biomarker levels among participants with a Conclusions: A score estimating the oral microbiome’s capacity for net NO production was associated with lower cardiometabolic risk biomarker levels only among those with lower nitrate intake.
Division:
Meeting: 2024 IADR/AADOCR/CADR General Session (New Orleans, Louisiana)
Location: New Orleans, Louisiana
Year: 2024
Final Presentation ID: 0895
Abstract Category|Abstract Category(s): Behavioral, Epidemiologic and Health Services Research
Authors
  • Goh, Charlene  ( National University of Singapore , Singapore , Singapore )
  • Colombo, Paolo  ( Department of Medicine,New York Presbyterian Hospital,Columbia University , New York , New York , United States )
  • Knight, Rob  ( University of California San Diego , La Jolla , California , United States )
  • Desvarieux, Moise  ( Mailman School of Public Health, Columbia University , New York , New York , United States ;  INSERM UMR 1153, Centre de Recherche Epidemiologie et Statistique Paris Sorbonne Cité (CRESS), METHODS Core , Paris , France )
  • Papapanou, Panos  ( College of Dental Medicine, Columbia University , New York , New York , United States )
  • Jacobs, David  ( School of Public Health,University of Minnesota , Minneapolis , Minnesota , United States )
  • Demmer, Ryan  ( School of Public Health,University of Minnesota , Minneapolis , Minnesota , United States ;  Mailman School of Public Health, Columbia University , New York , New York , United States ;  Department of Quantitative Health Sciences, Mayo Clinic, College of Medicine and Science , Rochester , Minnesota , United States )
  • Bohn, Bruno  ( School of Public Health,University of Minnesota , Minneapolis , Minnesota , United States )
  • Genkinger, Jeanine  ( Mailman School of Public Health, Columbia University , New York , New York , United States )
  • Molinsky, Rebecca  ( School of Public Health,University of Minnesota , Minneapolis , Minnesota , United States )
  • Roy, Sumith  ( Mailman School of Public Health, Columbia University , New York , New York , United States )
  • Paster, Bruce  ( The Forsyth Institute , Cambridge , Massachusetts , United States ;  Harvard School of Dental Medicine , Boston , Massachusetts , United States )
  • Chen, Ching-yuan  ( College of Dental Medicine, Columbia University , New York , New York , United States )
  • Rosenbaum, Michael  ( Departments of Pediatrics and Medicine, Columbia University , New York , New York , United States )
  • Yuzefpolskaya, Melana  ( Department of Medicine,New York Presbyterian Hospital,Columbia University , New York , New York , United States )
    • Support Funding Agency/Grant Number: National Institutes of Health (grants R00 DE018739, R21 DE022422, and R01 DK102932)
    Financial Interest Disclosure: NONE
    SESSION INFORMATION
    Poster Session
    Social and Environmental Factors and Oral Health
    Thursday, 03/14/2024 , 03:45PM - 05:00PM
    TABLES
    Estimated Mean Cardiometabolic Risk Biomarker (95% CI) Values among above vs. below Median MINES, Stratified by High vs. Low Dietary Nitrate Intake
     Low Dietary Nitrate Intake*  High Dietary Nitrate Intake*  p-value for interaction
     Below Median MINES** (n=179)Above Median MINES** (n=158)p-value***Below Median MINES**
    (n=158)
    		Above Median
    MINES**
    (n=180)
    		p-value*** 
    CMZ0.97
    (0.33, 1.60)
    		-0.12
    (-0.79, 0.55)
    		0.0020.07
    (-0.60, 0.73)

    		-0.09
    (-0.73, 0.54)
    		0.650.06
    Systolic Blood Pressure119
    (117, 121)
    		117
    (115, 119)
    		0.02
    		119
    (117, 121)
    		118
    (116, 120)
    		0.250.43
    Diastolic Blood Pressure73
    (72, 75)
    		72
    (70, 74)
    		0.0874
    (72, 75)
    		72
    (70, 74)
    		0.320.58
    HbA1c5.3
    (5.2, 5.3)
    		5.2
    (5.2, 5.3)
    		0.565.2
    (5.3, 5.3)
    		5.2
    (5.1, 5.3)
    		0.320.77
    Glucose86
    (85, 88)
    		86
    (84, 87)
    		0.2585
    (83, 86)
    		85
    (84, 87)
    		0.630.25
    Insulin6.9
    (6.2, 7.6)
    		5.9
    (5.3, 6.5)

    		0.0055.9
    (5.3, 6.5)
    		6.0
    (5.4, 6.6)
    		0.600.02
    HOMA-IR1.5
    (1.3, 1.6)
    		1.2
    (1.1, 1.4)
    		0.0051.2
    (1.1, 1.4)
    		1.2
    (1.1, 1.4)
    		0.620.02
    Multivariate linear regression models adjusted for total energy intake, sex, age, race/ethnicity, education, smoking status, body mass index, periodontal status and Alternative Healthy Eating Index *Median nitrate intake [25th,75th percentile] = 189 [72, 245] mg/day. **MINES: Microbiome Induced Nitric oxide Enrichment Score was calculated as a ratio of microbial gene abundances necessary for NO vs NH3 production. This score is a surrogate for enhanced net capacity for nitrate reduction and subsequent nitrite and nitric oxide generation. ***P-value for difference between groups with above vs. below median MINES within strata of Nitrate Intake.