IADR Abstract Archives
Role of Stannous Fluoride Dentifrice Stabilized With Nitrate and Phosphate in Reducing Bacterial Metabolism and Viability
Objectives: Stannous fluoride has long been recognized for its antibacterial properties and has been associated with helping to resolve gingivitis. Its efficacy however has been strongly correlated with the stability of the stannous ion. Protecting the oxidation of the metal is important in maintaining its efficacy. Real-time measurement of bacterial metabolic function following treatment with stannous toothpaste formulas were used to measure antibacterial performance.
The study examines how stannous fluoride's antibacterial efficacy, when combined with tetrasodium pyrophosphate (TSPP) and potassium nitrate for stabilization (SNaP), performs within a toothpaste and vs. other stabilized stannous toothpastes and a sodium fluoride control.
Methods: In this study, using an aged salivary biofilm model, we evaluate the immediate and long-lasting benefits of a SNaP formula (0.454% Stannous fluoride with potassium nitrate and TSPP) on bacterial bioenergetics (growth and metabolism) by measuring total oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in real-time in a Seahorse Extracellular Flux Analyzer (Agilent Technologies, Santa Clara, CA).
Results: When comparing SNaP to sodium fluoride toothpaste and other stannous fluoride toothpaste stabilized with sodium gluconate in the market, SNaP consistently demonstrated a significant reduction in OCR (p<0.05). This observation provides insights into how different stabilizing systems impact the stability of stannous fluoride and its ability to enhance the inhibition of bacterial respiration and energy generation within oral biofilms.
Bacterial production of acid is an indicator of glycolytic activity, and a lower pH indicates increased acidity, which can contribute to development of tooth decay. It's important to note that while other stannous-containing products exhibited a decrease in ECAR compared to sodium fluoride toothpaste, SNaP consistently displayed superior persistence and performance (p<0.05).
Conclusions: This study demonstrates that stabilization of Stannous with TSPP and potassium nitrate provides significant in vitro antibacterial efficacy from a toothpaste.
The study examines how stannous fluoride's antibacterial efficacy, when combined with tetrasodium pyrophosphate (TSPP) and potassium nitrate for stabilization (SNaP), performs within a toothpaste and vs. other stabilized stannous toothpastes and a sodium fluoride control.
Methods: In this study, using an aged salivary biofilm model, we evaluate the immediate and long-lasting benefits of a SNaP formula (0.454% Stannous fluoride with potassium nitrate and TSPP) on bacterial bioenergetics (growth and metabolism) by measuring total oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in real-time in a Seahorse Extracellular Flux Analyzer (Agilent Technologies, Santa Clara, CA).
Results: When comparing SNaP to sodium fluoride toothpaste and other stannous fluoride toothpaste stabilized with sodium gluconate in the market, SNaP consistently demonstrated a significant reduction in OCR (p<0.05). This observation provides insights into how different stabilizing systems impact the stability of stannous fluoride and its ability to enhance the inhibition of bacterial respiration and energy generation within oral biofilms.
Bacterial production of acid is an indicator of glycolytic activity, and a lower pH indicates increased acidity, which can contribute to development of tooth decay. It's important to note that while other stannous-containing products exhibited a decrease in ECAR compared to sodium fluoride toothpaste, SNaP consistently displayed superior persistence and performance (p<0.05).
Conclusions: This study demonstrates that stabilization of Stannous with TSPP and potassium nitrate provides significant in vitro antibacterial efficacy from a toothpaste.