IADR Abstract Archives
Bond Strength and Anti-Microbial of SDF With KI and STS
Objectives: Silver Diamine fluoride (SDF) is a clear, odorless liquid used to in the treatment of dental caries. Its use results in dark staining of carious enamel and dentin. Potassium Iodide (KI) and Sodium thiosulfate (STS) are two compounds that can decolorize the tooth structure. Our study evaluates the effect of using these decolorizers on the bond strength and antimicrobial properties of SDF.
Methods: With an in vitro experimental design, a total of 20 tooth specimens were divided into four treatment groups. The specimens were treated with SDF (Riva Star 1st step), SDF/KI (Riva Star 1st then 2nd Steps), SDF/STS (Riva Star 1st then STS 0.4g/mL), or left untreated (control). For antimicrobial test, the teeth were inoculated with Escherichia coli in 2% LB solution and incubated for 24 hours. Serial dilutions were performed and they were cultured for two days. The original clone forming units (CFUs) for each group were calculated. To test the shear bond strength, the tooth specimens after SDF treatment were etched with 35% phosphoric acid, primed with Scotchbond Universal adhesive, and were bonded to 3.5 mm diameter rod surface using Premium Flow Nano Composite (Henry Schein) with an Ultradent shear bond strength jigs. They were then tested using Instron 5566A with a 1kN load cell at 1mm/min crosshead speed. The tooth shade and color (L*a*b*) was measured using a Vita EasyShade V at pre-treatment, 1 day post treatment, 1 week post treatment, and 2 weeks post treatment. Color changes ΔE 2000 were calculated. The differences of SBS, CFU and ΔE 2000 among groups were analyzed one-way ANOVA Tukey test using JMP Pro 16.
Results: Table 1 shows the shear bond strength, Figure 1 shows the CFU after the treatment, and Table 2 shows the color difference after treatment.
Conclusions: No significantly difference in shear bond strength was found among the four groups. Samples treated with SDF with KI showed significantly less antimicrobial activity than SDF and SDF/STS. KI treatment showed a greater ability to decolorize SDF compared to STS.
Methods: With an in vitro experimental design, a total of 20 tooth specimens were divided into four treatment groups. The specimens were treated with SDF (Riva Star 1st step), SDF/KI (Riva Star 1st then 2nd Steps), SDF/STS (Riva Star 1st then STS 0.4g/mL), or left untreated (control). For antimicrobial test, the teeth were inoculated with Escherichia coli in 2% LB solution and incubated for 24 hours. Serial dilutions were performed and they were cultured for two days. The original clone forming units (CFUs) for each group were calculated. To test the shear bond strength, the tooth specimens after SDF treatment were etched with 35% phosphoric acid, primed with Scotchbond Universal adhesive, and were bonded to 3.5 mm diameter rod surface using Premium Flow Nano Composite (Henry Schein) with an Ultradent shear bond strength jigs. They were then tested using Instron 5566A with a 1kN load cell at 1mm/min crosshead speed. The tooth shade and color (L*a*b*) was measured using a Vita EasyShade V at pre-treatment, 1 day post treatment, 1 week post treatment, and 2 weeks post treatment. Color changes ΔE 2000 were calculated. The differences of SBS, CFU and ΔE 2000 among groups were analyzed one-way ANOVA Tukey test using JMP Pro 16.
Results: Table 1 shows the shear bond strength, Figure 1 shows the CFU after the treatment, and Table 2 shows the color difference after treatment.
Conclusions: No significantly difference in shear bond strength was found among the four groups. Samples treated with SDF with KI showed significantly less antimicrobial activity than SDF and SDF/STS. KI treatment showed a greater ability to decolorize SDF compared to STS.
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