IADR Abstract Archives
Biomechanically Advanced Polymer-Based Biocomposites for Oral and Maxillofacial Reconstruction
Objectives: The increased demand for implants but the availability of limited material types remain a substantial clinical challenge. Therefore, we aimed to develop a subverted traditional material that could be generalizable to any dental composite system while overcoming the limitations associated with conventional implants.
Methods: The resin-based biocomposites were synthesized by mixing 2 wt.% of nano-hydroxyapatites (nHAPs, D: 50-200 nm) and 10-20 wt.% of nano-glass particles (D<700nm) with photocurable dental monomer system (Figure 1A). The samples were randomly divided into a control and a test group with or without helium-oxygen cold plasma treatment, and tissue culture plates (TCPs) were used as a positive control. Mechanical properties were investigated through compression tests. In vitro cell proliferation with adherence, live/dead assay, and osteogenic differentiation including mineralization of MC3T3-E1 were evaluated while cytoskeleton structure was visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The in vivo study was performed on the left femur of 28 Sprague Dawley rats followed by histology analyzed via Toluidine Blue O staining.
Results: With the increased nano-fillers fraction, the mean value of the compressive strength and modulus increases from 104.04 to 121.25 MPa (16.5%) and from 2.18 to 3.41 GPa (56.4%), respectively (Figure 1B). After five days of cultivation of MC3T3-E1 on the plasma-treated surface, 99.35% viable cells were found (Figure 1C a). Cell adhesion and proliferation results revealed the treatment group showed higher values compared to the control group at 6 h and 5 d, respectively (Figure 1C b-c). The level of alkaline phosphatase remarkably increased from 7 to 14 d. The absorbance of Alizarin Red in the treatment group (2.90 ± 0.41) was significantly higher than in TCPS (3.02 ± 0.45) (p<0.05). Finally, new bony growth and its strong adherence on the implant surface was also observed in the femur defect model post 4- and 10-week implantation.
Conclusions: Collectively, the metal-free medical implants possessed strong mechanical strength, exhibited outstanding osteointegration activity, and its osteogenesis enhanced through cold plasma surface treatment.
Methods: The resin-based biocomposites were synthesized by mixing 2 wt.% of nano-hydroxyapatites (nHAPs, D: 50-200 nm) and 10-20 wt.% of nano-glass particles (D<700nm) with photocurable dental monomer system (Figure 1A). The samples were randomly divided into a control and a test group with or without helium-oxygen cold plasma treatment, and tissue culture plates (TCPs) were used as a positive control. Mechanical properties were investigated through compression tests. In vitro cell proliferation with adherence, live/dead assay, and osteogenic differentiation including mineralization of MC3T3-E1 were evaluated while cytoskeleton structure was visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). The in vivo study was performed on the left femur of 28 Sprague Dawley rats followed by histology analyzed via Toluidine Blue O staining.
Results: With the increased nano-fillers fraction, the mean value of the compressive strength and modulus increases from 104.04 to 121.25 MPa (16.5%) and from 2.18 to 3.41 GPa (56.4%), respectively (Figure 1B). After five days of cultivation of MC3T3-E1 on the plasma-treated surface, 99.35% viable cells were found (Figure 1C a). Cell adhesion and proliferation results revealed the treatment group showed higher values compared to the control group at 6 h and 5 d, respectively (Figure 1C b-c). The level of alkaline phosphatase remarkably increased from 7 to 14 d. The absorbance of Alizarin Red in the treatment group (2.90 ± 0.41) was significantly higher than in TCPS (3.02 ± 0.45) (p<0.05). Finally, new bony growth and its strong adherence on the implant surface was also observed in the femur defect model post 4- and 10-week implantation.
Conclusions: Collectively, the metal-free medical implants possessed strong mechanical strength, exhibited outstanding osteointegration activity, and its osteogenesis enhanced through cold plasma surface treatment.
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