IADR Abstract Archives
Benzyldimethyldodecyl Ammonium Chloride Doped Dental Denture-Based Resins: Impact on Strength, Surface Properties, Antifungal Activities
Objectives: This study examined the mechanical performance and antifungal properties of denture-based resins (DBRs) containing benzyldimethyldodecyl ammonium chloride (BDMDAC) as an antimicrobial compound.
Methods: A commercially available heat-polymerized acrylic DBR was modified to contain 3 or 5 wt.% of BDMDAC. The mechanical properties were evaluated using flexural strength (FS) and elastic modulus tests conducted by a universal testing machine. The microhardness and surface roughness (Ra, µm) of the specimens were recorded using Vicker’s microhardness and a non-contact optical profilometer, respectively. A 24-hour C. albicans biofilm was grown on the specimens and assessed via colony-forming units (CFUs) and scanning electron microscopy (SEM). In silico Molecular Docking was applied to predict the BDMDAC inhibition potential activity as an antifungal drug and its binding energies to two pathogenic enzymes, dihydrofolate reductase (DHFR) and candidapepsin-1 (SAPs) enzymes. One-way ANOVA and Tukey’s tests analyzed the data.
Results: The 3%-BDMDAC-DBR displayed excellent FS (66.67 ± 5.27 MPa), comparable to the control (74.51 ± 2.03 MPa), while the 5%-BDMDAC-DBR significantly (p=<0.05) decreased the FS (28.39 ± 7.63 MPa). No significant differences were found among the groups concerning their microhardness values (p>=0.05). Incorporating 3 and 5 wt.% of BDMDAC significantly reduced the DBR’s roughness compared to the control (p=<0.001). The C. albicans adhesion was significantly reduced (p=<0.001) by approximately 1.5 to 3-log in both the 3% and 5%-BDMDAC-DBR groups, respectively. The SEM images revealed fewer C. albicans colonies on the BDMDAC-DBR, with fewer morphological yeast-to-hyphal transitions compared to the control. Molecular docking results display a promising inhibition potential of BDMDAC as an antifungal drug. For DHFR and SAPs enzymes, BDMDAC illustrated binding energies equal to -5.3 and -5.5 kcal/mol, respectively.
Conclusions: 3%-BDMDAC-DBR demonstrated antifungal activities without deterioration effect on the mechanical performance, indicating its potential as a method for preventing and treating denture stomatitis.
Methods: A commercially available heat-polymerized acrylic DBR was modified to contain 3 or 5 wt.% of BDMDAC. The mechanical properties were evaluated using flexural strength (FS) and elastic modulus tests conducted by a universal testing machine. The microhardness and surface roughness (Ra, µm) of the specimens were recorded using Vicker’s microhardness and a non-contact optical profilometer, respectively. A 24-hour C. albicans biofilm was grown on the specimens and assessed via colony-forming units (CFUs) and scanning electron microscopy (SEM). In silico Molecular Docking was applied to predict the BDMDAC inhibition potential activity as an antifungal drug and its binding energies to two pathogenic enzymes, dihydrofolate reductase (DHFR) and candidapepsin-1 (SAPs) enzymes. One-way ANOVA and Tukey’s tests analyzed the data.
Results: The 3%-BDMDAC-DBR displayed excellent FS (66.67 ± 5.27 MPa), comparable to the control (74.51 ± 2.03 MPa), while the 5%-BDMDAC-DBR significantly (p=<0.05) decreased the FS (28.39 ± 7.63 MPa). No significant differences were found among the groups concerning their microhardness values (p>=0.05). Incorporating 3 and 5 wt.% of BDMDAC significantly reduced the DBR’s roughness compared to the control (p=<0.001). The C. albicans adhesion was significantly reduced (p=<0.001) by approximately 1.5 to 3-log in both the 3% and 5%-BDMDAC-DBR groups, respectively. The SEM images revealed fewer C. albicans colonies on the BDMDAC-DBR, with fewer morphological yeast-to-hyphal transitions compared to the control. Molecular docking results display a promising inhibition potential of BDMDAC as an antifungal drug. For DHFR and SAPs enzymes, BDMDAC illustrated binding energies equal to -5.3 and -5.5 kcal/mol, respectively.
Conclusions: 3%-BDMDAC-DBR demonstrated antifungal activities without deterioration effect on the mechanical performance, indicating its potential as a method for preventing and treating denture stomatitis.
4000724_File000000.jpg