IADR Abstract Archives
Salivary Proteome/Metabolome of Pregnant Women With Periodontitis and Obesity
Objectives: Obesity and pregnancy have synergistic effects on periodontal tissues, but the pathophysiological mechanisms behind these outcomes are still unclear. To better understand the biological mechanims related to the association of periodontitis and obesity during pregnancy, this study evaluated the proteins and metabolites in the saliva of pregnant women with/without periodontitis and/or obesity.
Methods: One hundred twenty-six pregnant women were consecutively recruited from Brazilian Primary Health Care, and after exclusion criteria they were divided into: obesity+periodontitis (OP); obesity/without periodontitis (OWP); normal BMI+periodontitis (NP); normal BMI/without periodontitis (NWP). Whole-Mouth Unstimulated Saliva was obtained from participants and individually processed. Metabolomics was performed by Proton Nuclear Magnetic Resonance (1H-NMR), operating at a proton frequency of 600.2 MHz at 298 K. Proteomics were performed by Mass Spectrometry coupled to liquid chromatography (nLC-ESI-MS/MS). Univariate and multivariate (PLS-DA, sPLS-DA, and VIP scores) analyses were performed based on metabolites concentrations and proteins expressions (p < 0.05).
Results: Greater metabolic dysregulation was found in pregnant women with periodontitis, regardless of the presence of obesity. OP and NP showed higher levels of leucine, valine, isoleucine, ornithine, and taurine (p < 0.001). The association of periodontitis and obesity altered the expression of several proteins, among them Submaxillary gland androgen-regulated protein 3B, S100-A8, MMP9, Heat shock 70kDa Protein 2 and 6, Putative HSP 70kDa Protein 7, HSP 71kDa, Haptoglobin, and Plastin-1 were up-regulated in OP (p > 0.05); while Cystatins (-S, -SA, -SN, -D), Lipocalin-1, Prolactin-induced protein, and Lactotransferrin were down-regulated in OP (p > 0.05).
Conclusions: In conclusion, periodontitis was more decisive in altering the salivary metabolome of pregnant women than obesity. Proteomic analysis, in turn, indicated that the association of periodontitis and obesity notably altered the salivary proteins during pregnancy, showing greater expression of pro-inflammatory proteins, related to individuals' immune and inflammatory responses, and lower expression of proteins with anti-proteolytic and antimicrobial activities.
Methods: One hundred twenty-six pregnant women were consecutively recruited from Brazilian Primary Health Care, and after exclusion criteria they were divided into: obesity+periodontitis (OP); obesity/without periodontitis (OWP); normal BMI+periodontitis (NP); normal BMI/without periodontitis (NWP). Whole-Mouth Unstimulated Saliva was obtained from participants and individually processed. Metabolomics was performed by Proton Nuclear Magnetic Resonance (1H-NMR), operating at a proton frequency of 600.2 MHz at 298 K. Proteomics were performed by Mass Spectrometry coupled to liquid chromatography (nLC-ESI-MS/MS). Univariate and multivariate (PLS-DA, sPLS-DA, and VIP scores) analyses were performed based on metabolites concentrations and proteins expressions (p < 0.05).
Results: Greater metabolic dysregulation was found in pregnant women with periodontitis, regardless of the presence of obesity. OP and NP showed higher levels of leucine, valine, isoleucine, ornithine, and taurine (p < 0.001). The association of periodontitis and obesity altered the expression of several proteins, among them Submaxillary gland androgen-regulated protein 3B, S100-A8, MMP9, Heat shock 70kDa Protein 2 and 6, Putative HSP 70kDa Protein 7, HSP 71kDa, Haptoglobin, and Plastin-1 were up-regulated in OP (p > 0.05); while Cystatins (-S, -SA, -SN, -D), Lipocalin-1, Prolactin-induced protein, and Lactotransferrin were down-regulated in OP (p > 0.05).
Conclusions: In conclusion, periodontitis was more decisive in altering the salivary metabolome of pregnant women than obesity. Proteomic analysis, in turn, indicated that the association of periodontitis and obesity notably altered the salivary proteins during pregnancy, showing greater expression of pro-inflammatory proteins, related to individuals' immune and inflammatory responses, and lower expression of proteins with anti-proteolytic and antimicrobial activities.