IADR Abstract Archives
The Small Phosphate Molecules Inducing Intrafibrillar Collagen Mineralization
Objectives: To investigate the intrafibrillar mineralization induced by small phosphate molecules --- sodium tripolyphosphate (TPP) without the action of the macromolecular polymers.
Methods: Single-layer reconstructed collagen model, 3D collagen model and demineralized dentin model were employed in this study. For the experimental group, 0.3M TPP solution was employed to pretreat for 20 min, and then rinsed with deionized water for 30 s. Sample was treated with deionized water as the control group. All samples were incubated in artificial saliva (AS) at 37 °C and then analyzed and recorded with transmission electron microscopy (TEM), fourier transform infrared spectrum (FTIR), ctyo-TEM, zeta potential, contact angle and 3D stochastic optical reconstruction microscopy (3D-STORM).
Results: After the pretreatment of TPP, the single-layer reconstructed collagen and 3D collagen film can be completely mineralized after incubation for 7 days. The remineralization of demineralized tooth can occur after 28 days. Cryo-TEM with tomography reveal the presence of crystalline minerals inside the TPP-collagen fibrils. However, the bare collagen fibrils were not mineralized. FTIR spectra of TPP-collagen showed that new peaks (O=P and P-O-C at 1030 cm−1 and 976 cm−1) derived from TPP. The surface potential of TPP-collagen (-18.62 ± 0.89 mV) was lower than that of bare collagen (-0.76 ± 0.87 mV, P < 0.05), the contact angle of TPP-collagen (77.98 ± 1.54°) was smaller than that of bare collagen (101.43 ± 1.51, P < 0.05). The 3D-STORM images reveal that TPP could be detected inside the collagen fibrils. With the increment of incubation time, more and more Ca2+ were distributed inside and on the surface of collagen.
Conclusions: Biomimetic mineralization could be induced by TPP after incubation in AS solution without the action of the macromolecular polymers. TPP-bound collagen can attract Ca2+or amorphous Ca/P precursors by electrostaticinteraction and then decrease its surface energy, promoting the nucleation of CaP.
Methods: Single-layer reconstructed collagen model, 3D collagen model and demineralized dentin model were employed in this study. For the experimental group, 0.3M TPP solution was employed to pretreat for 20 min, and then rinsed with deionized water for 30 s. Sample was treated with deionized water as the control group. All samples were incubated in artificial saliva (AS) at 37 °C and then analyzed and recorded with transmission electron microscopy (TEM), fourier transform infrared spectrum (FTIR), ctyo-TEM, zeta potential, contact angle and 3D stochastic optical reconstruction microscopy (3D-STORM).
Results: After the pretreatment of TPP, the single-layer reconstructed collagen and 3D collagen film can be completely mineralized after incubation for 7 days. The remineralization of demineralized tooth can occur after 28 days. Cryo-TEM with tomography reveal the presence of crystalline minerals inside the TPP-collagen fibrils. However, the bare collagen fibrils were not mineralized. FTIR spectra of TPP-collagen showed that new peaks (O=P and P-O-C at 1030 cm−1 and 976 cm−1) derived from TPP. The surface potential of TPP-collagen (-18.62 ± 0.89 mV) was lower than that of bare collagen (-0.76 ± 0.87 mV, P < 0.05), the contact angle of TPP-collagen (77.98 ± 1.54°) was smaller than that of bare collagen (101.43 ± 1.51, P < 0.05). The 3D-STORM images reveal that TPP could be detected inside the collagen fibrils. With the increment of incubation time, more and more Ca2+ were distributed inside and on the surface of collagen.
Conclusions: Biomimetic mineralization could be induced by TPP after incubation in AS solution without the action of the macromolecular polymers. TPP-bound collagen can attract Ca2+or amorphous Ca/P precursors by electrostaticinteraction and then decrease its surface energy, promoting the nucleation of CaP.