IADR Abstract Archives
Effect of MaquiCPI-3 Against Initial Erosion on Enamel and Dentin
Objectives: The aim of this study was to analyze the preventive effect and define the best concentration of a new recombinant cystatin derived from Maqui Berry (MaquiCPI-3) for the prevention of dentin and enamel erosion in vitro.
Methods: MaquiCPI-3 was produced recombinantly through the E. coli expression system. Two-hundred and eighty specimens were prepared (140 bovine enamel and 140 dentin bovine), randomized and distributed into 7 groups (n/group=20): Deionized water (negative control), 0.1mg/mL CaneCPI-5 (positive control 1), Elmex® Erosion (positive control 2) and four experimental groups (0.1mg/mL MaquiCPI-3, 0.25mg/mL MaquiCPI-3, 0.5mg/mL MaquiCPI-3 and 1.0mg/mL MaquiCPI-3). Initially, the samples were treated (250µL) with the respective solutions for 2h, 37°C. Then, the acquired pellicle was formed (250µL of stimulated human saliva) for 2h, 37°C. Subsequently, the erosive challenge was performed (1mL of 0.65% citric acid, pH3.4, for 1min, 25°C. Surface microhardness (SM) analysis was performed and the percentage of surface microhardness change (%SMC) was calculated. Data were analyzed by ANOVA/Tukey (p<0.05).
Results: For enamel, specimens treated with Water presented significantly greater mean(±SD) %SMC (37.56%±13.98) compared to the other treatments: Elmex® (22.30%±10.06), 0.1mg/mL CaneCPI-5 (19.37%±10.41), 0.1mg/mL MaquiCPI-3 (19.93%±10.26), 0.25mg/mL MaquiCPI-3 (23.72%±9.74) and 1.0mg/mL MaquiCPI-3 (19.23%±12.01). In addition, 0.5mg/mL MaquiCPI-3 (2.90%±3.35) demonstrated the best protective effect (significantly) compared to the other treatments. For dentin, Water (38.38%±8.97), Elmex® (33.25%±5.62) and 1.0mg/mL MaquiCPI-3 (35,77%±10.24) presented significantly greater %SMC in comparison to the treatments with 0.1mg/mL CaneCPI-5 (10.49%±5.57), 0.1mg/mL MaquiCPI-3 (14.87%±10.18), 0.25mg/mL MaquiCPI-3 (18.52%±10.33) and 0.5mg/mL MaquiCPI-3 (9.76%±10.93), which presented the lowest %SMC. In conclusion, at 0.5mg/mL MaquiCPI-3 obtained the best protection for the enamel, while the lowest concentrations of MaquiCPI-3 (0.1-0.5mg/mL) showed protection for the dentin.
Conclusions: In conclusion, at 0.5mg/mL MaquiCPI-3 obtained the best protection for the enamel, while the lowest concentrations of MaquiCPI-3 (0.1-0.5mg/mL) showed protection for the dentin.These results open a new avenue for the development of dental products using MaquiCPI-3 to protect against enamel and dentin erosion.
Methods: MaquiCPI-3 was produced recombinantly through the E. coli expression system. Two-hundred and eighty specimens were prepared (140 bovine enamel and 140 dentin bovine), randomized and distributed into 7 groups (n/group=20): Deionized water (negative control), 0.1mg/mL CaneCPI-5 (positive control 1), Elmex® Erosion (positive control 2) and four experimental groups (0.1mg/mL MaquiCPI-3, 0.25mg/mL MaquiCPI-3, 0.5mg/mL MaquiCPI-3 and 1.0mg/mL MaquiCPI-3). Initially, the samples were treated (250µL) with the respective solutions for 2h, 37°C. Then, the acquired pellicle was formed (250µL of stimulated human saliva) for 2h, 37°C. Subsequently, the erosive challenge was performed (1mL of 0.65% citric acid, pH3.4, for 1min, 25°C. Surface microhardness (SM) analysis was performed and the percentage of surface microhardness change (%SMC) was calculated. Data were analyzed by ANOVA/Tukey (p<0.05).
Results: For enamel, specimens treated with Water presented significantly greater mean(±SD) %SMC (37.56%±13.98) compared to the other treatments: Elmex® (22.30%±10.06), 0.1mg/mL CaneCPI-5 (19.37%±10.41), 0.1mg/mL MaquiCPI-3 (19.93%±10.26), 0.25mg/mL MaquiCPI-3 (23.72%±9.74) and 1.0mg/mL MaquiCPI-3 (19.23%±12.01). In addition, 0.5mg/mL MaquiCPI-3 (2.90%±3.35) demonstrated the best protective effect (significantly) compared to the other treatments. For dentin, Water (38.38%±8.97), Elmex® (33.25%±5.62) and 1.0mg/mL MaquiCPI-3 (35,77%±10.24) presented significantly greater %SMC in comparison to the treatments with 0.1mg/mL CaneCPI-5 (10.49%±5.57), 0.1mg/mL MaquiCPI-3 (14.87%±10.18), 0.25mg/mL MaquiCPI-3 (18.52%±10.33) and 0.5mg/mL MaquiCPI-3 (9.76%±10.93), which presented the lowest %SMC. In conclusion, at 0.5mg/mL MaquiCPI-3 obtained the best protection for the enamel, while the lowest concentrations of MaquiCPI-3 (0.1-0.5mg/mL) showed protection for the dentin.
Conclusions: In conclusion, at 0.5mg/mL MaquiCPI-3 obtained the best protection for the enamel, while the lowest concentrations of MaquiCPI-3 (0.1-0.5mg/mL) showed protection for the dentin.These results open a new avenue for the development of dental products using MaquiCPI-3 to protect against enamel and dentin erosion.