IADR Abstract Archives
Ion Bioavailability and Remineralisation With Stannous Fluoride-Containing Dentifrices in Situ
Objectives: Modern dentifrices contain SnF2 for a range of benefits including periodontal health. It is important that these formulations release bioavailable Sn and F ions to promote remineralisation. The aim of this blinded, crossover in situ clinical trial was to compare Sn and F ion release and enamel and dentine remineralisation by two SnF2-containing dentifrices.
Methods: Six participants wore removal palatal appliances holding human enamel and dentine blocks with subsurface lesions. Appliances were worn for two treatment periods of 14 consecutive days each, with a one-week washout period in-between. Participants were randomly allocated to rinse with a 1:4 diluted slurry of one of two dentifrices containing either 5% casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) + 1100 ppm F as SnF2 (MI Paste One Perio [MIPOP], GC Corporation) or 1100 ppm F as SnF2 Colgate TotalSF [CT], Colgate-Palmolive USA) for 1 min, four times a day. Saliva was collected post-treatment and analysed for Sn and F ions using atomic absorption spectrophotometry and ion chromatography, respectively. Enamel and dentine blocks were removed, paired with untreated controls, and analysed for percent remineralisation (%R) using transverse microradiography (TMR) and percent surface microhardness recovery (%SMHR).
Results: MIPOP released 3.00 ± 0.25 mM F and 1.29 ± 0.21 mM Sn into saliva whereas CT released 2.89 ± 0.30 mM F and only 0.28 ± 0.17 mM Sn. MIPOP produced significantly higher %R than CT: 25.6 ± 1.9% compared to 15.2 ± 1.0% in enamel, and 33.6 ± 2.9% compared to 20.6 ± 1.0% in dentine. Additionally, MIPOP produced significantly higher %SMHR (15.5 ± 8.4%) compared to CT (4.1 ± 3.0%).
Conclusions: Both dentifrices promoted remineralisation, but the MIPOP dentifrice with added 5% CPP-ACP and the ion-stabilising effects of CPP released higher amounts of Sn ions and produced significantly higher remineralisation.
Methods: Six participants wore removal palatal appliances holding human enamel and dentine blocks with subsurface lesions. Appliances were worn for two treatment periods of 14 consecutive days each, with a one-week washout period in-between. Participants were randomly allocated to rinse with a 1:4 diluted slurry of one of two dentifrices containing either 5% casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) + 1100 ppm F as SnF2 (MI Paste One Perio [MIPOP], GC Corporation) or 1100 ppm F as SnF2 Colgate TotalSF [CT], Colgate-Palmolive USA) for 1 min, four times a day. Saliva was collected post-treatment and analysed for Sn and F ions using atomic absorption spectrophotometry and ion chromatography, respectively. Enamel and dentine blocks were removed, paired with untreated controls, and analysed for percent remineralisation (%R) using transverse microradiography (TMR) and percent surface microhardness recovery (%SMHR).
Results: MIPOP released 3.00 ± 0.25 mM F and 1.29 ± 0.21 mM Sn into saliva whereas CT released 2.89 ± 0.30 mM F and only 0.28 ± 0.17 mM Sn. MIPOP produced significantly higher %R than CT: 25.6 ± 1.9% compared to 15.2 ± 1.0% in enamel, and 33.6 ± 2.9% compared to 20.6 ± 1.0% in dentine. Additionally, MIPOP produced significantly higher %SMHR (15.5 ± 8.4%) compared to CT (4.1 ± 3.0%).
Conclusions: Both dentifrices promoted remineralisation, but the MIPOP dentifrice with added 5% CPP-ACP and the ion-stabilising effects of CPP released higher amounts of Sn ions and produced significantly higher remineralisation.