IADR Abstract Archives
Buffalo Fluoride Laser Odontoplasty: a Novel Approach to Caries Prevention
Objectives: Dental Caries is a multifactorial disease and many recent advances have focused on oral health preventive measures. Laser technology has revolutionized the field of dentistry through its wide range of applications. We utilized lasers for two discrete applications aimed at reduction of the occlusal surface complexity and increasing fluoride incorporation.
Methods: Deidentified extracted human teeth were collected under UB IRB protocol (##00001193), sectioned and embedded in a polymer block. In the first part of the study, occlusal surfaces were treated with a dental laser at various settings, and reduction in surface complexity was assessed with microcomputed tomography and dye retention following simulated mechanical brushing. For the second phase, Silver Diamine Fluoride (SDF) varnish and various laser wavelengths (447 nm, 685 nm, 810 nm, 940 nm, 980 nm, and 1064 nm) were used. Samples were then subjected to acid demineralization study in Lactic Acid (10.2 M) solution for five days in an incubator at 37 °C with mechanical agitations and samples were collected at day 0, 1, 3, and 5. Colorimetric assays (Arsenazo and Malachite Green) were performed with a spectrophotometer (Molecular Devices). Confirmatory analysis and techniques like DSC-TGA, FTIR and SEM are being used to assess the precise degree of substitution.
Results: Laser odontoplasty significantly (n = 18, p less 0.05) reduced surface complexity and increased self-cleansing occlusal areas. The combination of a red laser (658 nm at 30 mW/cm2 for 54 sec at 1.62 J/cm2, 3 p.J/cm2 0.7 Einstein) with SDF application significantly (n = 2, p less 0.05) reduced acid demineralization compared to SDF alone.
Conclusions: The novel Buffalo fluoride laser Odontoplasty (BFLO) technique demonstrated reduction in the occlusal retentive surfaces and significant improvement in resistance to acid dimeneralization. This combined treatment approach can significantly prevent childhood caries and can have a potential impact on clinical and public health dentistry.
Methods: Deidentified extracted human teeth were collected under UB IRB protocol (##00001193), sectioned and embedded in a polymer block. In the first part of the study, occlusal surfaces were treated with a dental laser at various settings, and reduction in surface complexity was assessed with microcomputed tomography and dye retention following simulated mechanical brushing. For the second phase, Silver Diamine Fluoride (SDF) varnish and various laser wavelengths (447 nm, 685 nm, 810 nm, 940 nm, 980 nm, and 1064 nm) were used. Samples were then subjected to acid demineralization study in Lactic Acid (10.2 M) solution for five days in an incubator at 37 °C with mechanical agitations and samples were collected at day 0, 1, 3, and 5. Colorimetric assays (Arsenazo and Malachite Green) were performed with a spectrophotometer (Molecular Devices). Confirmatory analysis and techniques like DSC-TGA, FTIR and SEM are being used to assess the precise degree of substitution.
Results: Laser odontoplasty significantly (n = 18, p less 0.05) reduced surface complexity and increased self-cleansing occlusal areas. The combination of a red laser (658 nm at 30 mW/cm2 for 54 sec at 1.62 J/cm2, 3 p.J/cm2 0.7 Einstein) with SDF application significantly (n = 2, p less 0.05) reduced acid demineralization compared to SDF alone.
Conclusions: The novel Buffalo fluoride laser Odontoplasty (BFLO) technique demonstrated reduction in the occlusal retentive surfaces and significant improvement in resistance to acid dimeneralization. This combined treatment approach can significantly prevent childhood caries and can have a potential impact on clinical and public health dentistry.