IADR Abstract Archives
Tooth Regeneration in rat Model: a Pilot Study Part I
Objectives: Determinate tooth germ developmental stage (cap stage) of Wistar rat according to embryonic day.
Methods: Three albino Wistar rats with different days of gestation were used; one from 14 – 15 days, another from 15 - 16 days and 17 – 18 days of gestation. Euthanasia and hysterectomy were performed and the fetuses were extracted. Each sample was stored in a 1ml vial with 10% formaldehyde for later histological study by staining with H&E stain.
Results: A total of 35 fetuses was used to determine developmental stage. In 13-14 ED fetuses ware no tooth germs founded. Bud stage tooth germs were founded in days 15-16 ED fetuses and cap stage tooth germs were founded in 17-18 ED fetuses.
Conclusions: Tooth germs cap stage for mice is 14.5 ED, while 17-18 ED for rat Wistar. In our pilot study has shown that odontogénesis stage in rat Wistar fetus and mice fetus are different.
Methods: Three albino Wistar rats with different days of gestation were used; one from 14 – 15 days, another from 15 - 16 days and 17 – 18 days of gestation. Euthanasia and hysterectomy were performed and the fetuses were extracted. Each sample was stored in a 1ml vial with 10% formaldehyde for later histological study by staining with H&E stain.
Results: A total of 35 fetuses was used to determine developmental stage. In 13-14 ED fetuses ware no tooth germs founded. Bud stage tooth germs were founded in days 15-16 ED fetuses and cap stage tooth germs were founded in 17-18 ED fetuses.
Conclusions: Tooth germs cap stage for mice is 14.5 ED, while 17-18 ED for rat Wistar. In our pilot study has shown that odontogénesis stage in rat Wistar fetus and mice fetus are different.
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