IADR Abstract Archives
Characterization and Application of a Lactobacilli-Postbiotic on Streptococcus Mutans Biofilm
Objectives: Postbiotic is the term used to define the soluble factors, metabolic products, or by-products released by live probiotic bacteria or after it lysis. This study aimed to perform the chemical characterization of the postbiotic of Lacticaseibacillus rhamnosus LR-32 and to evaluate its effect on the development of the Streptococcus mutans biofilm.
Methods: After the cultivation of the probiotic strain, the postbiotic was extracted by centrifuging the culture and filtering the supernatant. This postbiotic was used to perform its chemical characterization using Gas Chromatography coupled with mass spectrometry, determine the growth inhibition of Streptococcus mutans in its planktonic form, and evaluate the following parameters of 48h-Streptococcus mutans biofilms: viable bacteria, dry-weight and gene expression of glucosyltransferases and VicR gene. The control group consisted of the Streptococcus mutans biofilm without any treatment. A paired t-test was performed for statistical analysis with p set at 5%.
Results: Seventeen compounds were identified in the postbiotic of Lacticaseibacillus rhamnosus LR-32 of various chemical classes, including sugars, amino acids, vitamins, and acids. The treatment with the postbiotic led to an inhibition of the growth of Streptococcus mutans in its planktonic form, as well as a decrease in the number of viable bacteria, reduction of dry-weight, and negatively regulated the gene expression of gtfB, gtfC, gtfD and vicR in Streptococcus mutans biofilms compared with the non-treated biofilm (p < 0,05).
Conclusions: Thus, the most abundant compounds in the postbiotic of Lacticaseibacillus rhamnosus LR-32 corresponded to citric acid, silanol, and β-D-glucopyranose and their application in a in vitro biofilm model interfered with the growth and glucan production of Streptococcus mutans.
Methods: After the cultivation of the probiotic strain, the postbiotic was extracted by centrifuging the culture and filtering the supernatant. This postbiotic was used to perform its chemical characterization using Gas Chromatography coupled with mass spectrometry, determine the growth inhibition of Streptococcus mutans in its planktonic form, and evaluate the following parameters of 48h-Streptococcus mutans biofilms: viable bacteria, dry-weight and gene expression of glucosyltransferases and VicR gene. The control group consisted of the Streptococcus mutans biofilm without any treatment. A paired t-test was performed for statistical analysis with p set at 5%.
Results: Seventeen compounds were identified in the postbiotic of Lacticaseibacillus rhamnosus LR-32 of various chemical classes, including sugars, amino acids, vitamins, and acids. The treatment with the postbiotic led to an inhibition of the growth of Streptococcus mutans in its planktonic form, as well as a decrease in the number of viable bacteria, reduction of dry-weight, and negatively regulated the gene expression of gtfB, gtfC, gtfD and vicR in Streptococcus mutans biofilms compared with the non-treated biofilm (p < 0,05).
Conclusions: Thus, the most abundant compounds in the postbiotic of Lacticaseibacillus rhamnosus LR-32 corresponded to citric acid, silanol, and β-D-glucopyranose and their application in a in vitro biofilm model interfered with the growth and glucan production of Streptococcus mutans.