Cytotoxicity of Novel Fluorescent Dyes

Objectives:
The aim of this research is to test the intracellular binding and cytotoxicity of newly developed florescent dyes in adult stem cells from apical papilla (SCAP)
Methods: The experiments were conducted with SCAP passage 4. The cells were cultured at standart conditions and after reaching 90% confluence were seeded on 24-well plate for further processing.
New 2-methyl-benzothiazol dies were administered in final concentrations of 2, 20, 200 μM.
Cellular penetration was observed and recorded on Incell Analyzer 6000 (GE HealthCare).
Anexin V test was used for evaluating apoptotic cells 24h after treatment.
For High through-put florescent cellular analysis, the apoptotic cells were visualized with FITC- conjugated anti-annexine V antibody. The nuclei were counterstained with DAPI.
Results: The dyes pentrated the living cells with specific binding to granular EPR. No cytotoxic effects to the cells were observed. Cellular proliferation was not affected with the administered doses.
Conclusions: Newly synthesised dyes proved to be promising for future application dew to limited or no cytotoxicity.