IADR Abstract Archives
Biocompatible Effect of Magnesium Oxide on Human Dental Pulp Cells
Objectives: Magnesium has attracted increasing attention as a promising class of biomaterials for dental medicine. However, the effect of magnesium oxide (MgO) on biocompatibility of human dental pulp cells (HDPCs) has not been investigated. This study was designed to evaluate the effect of different concentrations of MgO on cellular viability of HDPCs
Methods: HDPCs were cultured in six replicates with 0.5mM, 1mM, 2mM, 4mM, 8mM supplemental MgO, 0 mM as the negative control group, lignin sulfonic acid sodium salt and xanthan gum as the vehicle control groups. Cellular viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. HDPCs were seeded at a density of 300 cells per well. Cellular activity was assessed by measuring mitochondrial succinic dehydrogenase (SDH) activity at 16 hrs, 3, 7, 10 and 14 days. The absorbance reading of the produced purple formazan of each group was recorded at wavelength 590 nm. Statistical analysis was performed using multi-way ANOVA with Wilks’ lambda test.
Results: At 16 hrs, the 0.5 mM supplemental MgO concentration group showed a SDH activity value of 44.50%, followed by 73.58 % at day 3, 81.07% at day 7, 84.06% at day 10 and 92.98% at day 14, showing SDH activity values equivalent to the negative control and vehicle control groups (P<0.001 ). However, for the 1mM, 2 mM, 4 mM and 8mM supplemental MgO concentrations, SDH activity showed significant decrease at 16 hrs, days 3, 7, 10 and 14 days in comparison with the negative control and vehicle control groups (P<0.001)
Conclusions: Optimal concentration of MgO (0.5mM) could be biocompatible to HDPCs, while MgO with higher concentrations could be toxic to HDPCs.
Methods: HDPCs were cultured in six replicates with 0.5mM, 1mM, 2mM, 4mM, 8mM supplemental MgO, 0 mM as the negative control group, lignin sulfonic acid sodium salt and xanthan gum as the vehicle control groups. Cellular viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. HDPCs were seeded at a density of 300 cells per well. Cellular activity was assessed by measuring mitochondrial succinic dehydrogenase (SDH) activity at 16 hrs, 3, 7, 10 and 14 days. The absorbance reading of the produced purple formazan of each group was recorded at wavelength 590 nm. Statistical analysis was performed using multi-way ANOVA with Wilks’ lambda test.
Results: At 16 hrs, the 0.5 mM supplemental MgO concentration group showed a SDH activity value of 44.50%, followed by 73.58 % at day 3, 81.07% at day 7, 84.06% at day 10 and 92.98% at day 14, showing SDH activity values equivalent to the negative control and vehicle control groups (P<0.001 ). However, for the 1mM, 2 mM, 4 mM and 8mM supplemental MgO concentrations, SDH activity showed significant decrease at 16 hrs, days 3, 7, 10 and 14 days in comparison with the negative control and vehicle control groups (P<0.001)
Conclusions: Optimal concentration of MgO (0.5mM) could be biocompatible to HDPCs, while MgO with higher concentrations could be toxic to HDPCs.