IADR Abstract Archives
Etchants and Plant-Based Polyphenols: Combined Effect on Long-Term Resin-Dentin Bonds
Objectives: This study investigated the combined effect of dentin etchants [i.e., phosphoric acid (PA) and ethylenediaminetetraacetic acid (EDTA)] and plant-based polyphenols (PBPs) [luteolin (LT), galangin (GL), and proanthocyanidin (PAC)] on the stability of resin-dentin bonds.
Methods: Thirty-two non-carious, extracted human third molars were collected. The occlusal surface was removed using a low-speed diamond rotary saw under water-cooling to expose a flat dentin surface subsequently polished through 600 grit paper. Samples were randomized to be etched with either 37% PA (15s) or 18% EDTA (60s), and again randomized for treatment with one of four priming solutions: 1) distilled water (W, -control); 2) 0.2% LT; 3) 0.4% GL, or 4) 1% PAC (+control). Primers were scrubbed on PA-etched or EDTA-etched dentin for 30s and any excess removed. Adhesive ExciTE-F (Ivoclar, Armhest, NY, USA) was applied over primed surfaces (20s), air-dried (5s) and light-cured (10s) (VALO, Ultradent Products, South Jordan, UT; irradiance>1200mw/cm2). Composite (Empress-Direct, Ivoclar) buildups were made with four, 1.5mm increments and polymerized (20s). Samples (n=5 teeth/group) were stored in ultra-purified water (37oC/24hr), sectioned into beams for microtensile testing (µTBS), and tested either immediately (IM) or after a 12-month water storage (12-mon) using a universal testing machine (EZ-LX, Shimadzu, Columbia, MD, USA). Data were analyzed by ANOVA (post-hoc Tukey, α=0.05).
Results: There was no significant difference between immediate and 12-month bond strengths for any plant-based polyphenol tested with either etchant. While there was a significant decrease in PA-etched, water-primed samples, this was not true for EDTA-etched samples. While not significant, there was some bond strength reduction for PA-etched PBPs over time, while bond strengths for EDTA-etched PBPs were maintained.
Conclusions: Results indicate there is a synergistic effect of EDTA-etching and treatment with plant-based polyphenols that stabilizes bond strength in etch-and-rinse adhesives to dentin at least one year following placement.
Methods: Thirty-two non-carious, extracted human third molars were collected. The occlusal surface was removed using a low-speed diamond rotary saw under water-cooling to expose a flat dentin surface subsequently polished through 600 grit paper. Samples were randomized to be etched with either 37% PA (15s) or 18% EDTA (60s), and again randomized for treatment with one of four priming solutions: 1) distilled water (W, -control); 2) 0.2% LT; 3) 0.4% GL, or 4) 1% PAC (+control). Primers were scrubbed on PA-etched or EDTA-etched dentin for 30s and any excess removed. Adhesive ExciTE-F (Ivoclar, Armhest, NY, USA) was applied over primed surfaces (20s), air-dried (5s) and light-cured (10s) (VALO, Ultradent Products, South Jordan, UT; irradiance>1200mw/cm2). Composite (Empress-Direct, Ivoclar) buildups were made with four, 1.5mm increments and polymerized (20s). Samples (n=5 teeth/group) were stored in ultra-purified water (37oC/24hr), sectioned into beams for microtensile testing (µTBS), and tested either immediately (IM) or after a 12-month water storage (12-mon) using a universal testing machine (EZ-LX, Shimadzu, Columbia, MD, USA). Data were analyzed by ANOVA (post-hoc Tukey, α=0.05).
Results: There was no significant difference between immediate and 12-month bond strengths for any plant-based polyphenol tested with either etchant. While there was a significant decrease in PA-etched, water-primed samples, this was not true for EDTA-etched samples. While not significant, there was some bond strength reduction for PA-etched PBPs over time, while bond strengths for EDTA-etched PBPs were maintained.
Conclusions: Results indicate there is a synergistic effect of EDTA-etching and treatment with plant-based polyphenols that stabilizes bond strength in etch-and-rinse adhesives to dentin at least one year following placement.
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