IADR Abstract Archives
Cytotoxicity, Genotoxicity and Antimicrobial Profile of Biosynthesized Metal Nanoparticle
Objectives: To evaluate the cytotoxicity, genotoxicity and antimicrobial profile of silver nanoparticles (AgNPs) synthesized using Pelargonium hortorum.
Methods: Biosynthesis of AgNPs was carried out using Pelargonium-hortorum. Optical and morphological characterizations were studied by UV-Vis and TEM. Based on the synthesis concentration and time, various groups of resulting NPs were analyzed. Agar-diffusion tests were performed using Streptococcus-mutans. AgNPs concentration was maintained at 420 μg/mL and the inhibition-zone (ZoI) was measured. Both microdilution and cytotoxicity assays were carried out to determine MIC and CC50 respectively at different time intervals. Finally, based on these results genotoxicity tests were carried out with a qualitative analysis of HGF cell nuclei.
Results: Semi-spherical NPs morphology was observed in all groups. The particle size and UV-Vis absorbance were in the range of 8-65nm and 408-431nm respectively. Group-A:29.4±9.4nm & 420nm, Group-B:36.2±15.1nm & 423nm, Group-C:26.7±14.8nm & 431nm, Group-D:24.2±9.1nm & 412nm. Group E: 40.2±24.6 nm & 415 nm, Group F:35.2±10.7nm & 419nm, and Group G: 8.3±8.2 & 408nm. In the antimicrobial study, the ZoI was in the range of 6-26mm for different groups. Group-A:9.6±0.5mm, Group-B:6.3±0.5mm, Group-C:7.3±0.5mm, Group-D:11.5±0.5mm, Group E:13.0±0.5 mm, Group F:13.3±0.5 mm and Group G:8.3±0.5 mm, showing a significant difference (p≤0.001). The MIC was found around 250-400μg/mL. In relation to cytotoxicity, dose time-dependent effect was observed resulting in CC50 of 2.33, 2.38, 2.23 and 4.51 μg/mL at 1, 3, 6 and 24 h. The genotoxicity results using HGF cells showed that the nuclei cells were intact without any damage even after the incubation of biosynthesized AgNPs.
Conclusions: From the study, we conclude that the AgNPs of 35 nm and a precursor concentration of 1200 μL showed a greater antimicrobial effect. Thus, our results proved that the biosynthesized NPs showed no cytotoxicity and most importantly no negative effects to the nuclei cells.
Methods: Biosynthesis of AgNPs was carried out using Pelargonium-hortorum. Optical and morphological characterizations were studied by UV-Vis and TEM. Based on the synthesis concentration and time, various groups of resulting NPs were analyzed. Agar-diffusion tests were performed using Streptococcus-mutans. AgNPs concentration was maintained at 420 μg/mL and the inhibition-zone (ZoI) was measured. Both microdilution and cytotoxicity assays were carried out to determine MIC and CC50 respectively at different time intervals. Finally, based on these results genotoxicity tests were carried out with a qualitative analysis of HGF cell nuclei.
Results: Semi-spherical NPs morphology was observed in all groups. The particle size and UV-Vis absorbance were in the range of 8-65nm and 408-431nm respectively. Group-A:29.4±9.4nm & 420nm, Group-B:36.2±15.1nm & 423nm, Group-C:26.7±14.8nm & 431nm, Group-D:24.2±9.1nm & 412nm. Group E: 40.2±24.6 nm & 415 nm, Group F:35.2±10.7nm & 419nm, and Group G: 8.3±8.2 & 408nm. In the antimicrobial study, the ZoI was in the range of 6-26mm for different groups. Group-A:9.6±0.5mm, Group-B:6.3±0.5mm, Group-C:7.3±0.5mm, Group-D:11.5±0.5mm, Group E:13.0±0.5 mm, Group F:13.3±0.5 mm and Group G:8.3±0.5 mm, showing a significant difference (p≤0.001). The MIC was found around 250-400μg/mL. In relation to cytotoxicity, dose time-dependent effect was observed resulting in CC50 of 2.33, 2.38, 2.23 and 4.51 μg/mL at 1, 3, 6 and 24 h. The genotoxicity results using HGF cells showed that the nuclei cells were intact without any damage even after the incubation of biosynthesized AgNPs.
Conclusions: From the study, we conclude that the AgNPs of 35 nm and a precursor concentration of 1200 μL showed a greater antimicrobial effect. Thus, our results proved that the biosynthesized NPs showed no cytotoxicity and most importantly no negative effects to the nuclei cells.