IADR Abstract Archives
TNF-α and TGF-β in Current and Past Areca nut Users
Objectives: The arecoline in Areca Nut (AN) can induce pro-oncogenic cytokines like TNF-α and TGF-β that can lead to chronic inflammation, fibrotic changes and malignant transformation in AN users. The main objective of this study was to determine and compare the oral secretory concentration of TNF-α and TGF-β in current and past AN users
Methods: A prospective observational study where 80 subjects with self –reported AN habits (> 6 months) [40 Current AN users (CAN); 40 Past AN users (PAN)], with/ without AN associated oral mucosal lesions were recruited after ethical clearance and informed consent. History of current/past malignancy, uncontrolled systemic disease, acute/chronic inflammatory disorders were exclusion criteria. CAN underwent behavioral counseling for habit cessation. The subjects were asked to abstain from eating, drinking and rinsing the mouth at least 2 hours prior to sampling. Two sterile ophthalmic sponges, pre- wet with normal saline were kept in contact with the lesion/ buccal mucosa without rubbing or movement for 1 minute each, to collect the oral secretory cytokines and immediately stored in sterile containers at -80 °C till further analysis by ELISA.
Results: The concentration of TNF-α was significantly higher in PAN (p≤0.0342), while there was no significant difference in TGF-β levels between the two groups (p≤0.7737). The levels of TNF-α were higher in subjects with OSMF in both the groups.
Conclusions: Pro- oncogenic cytokines like TNF-α are significantly raised in PAN and in those with OSMF even after cessation of the AN habit. Role of TNF-α inhibitors in prevention of oral carcinogenesis in both CAN and PAN needs to be investigated. Although habit abstinence is imperative for risk reduction of oral cancer in AN users, regular surveillance for malignant transformation is required in both current and past AN users.
Methods: A prospective observational study where 80 subjects with self –reported AN habits (> 6 months) [40 Current AN users (CAN); 40 Past AN users (PAN)], with/ without AN associated oral mucosal lesions were recruited after ethical clearance and informed consent. History of current/past malignancy, uncontrolled systemic disease, acute/chronic inflammatory disorders were exclusion criteria. CAN underwent behavioral counseling for habit cessation. The subjects were asked to abstain from eating, drinking and rinsing the mouth at least 2 hours prior to sampling. Two sterile ophthalmic sponges, pre- wet with normal saline were kept in contact with the lesion/ buccal mucosa without rubbing or movement for 1 minute each, to collect the oral secretory cytokines and immediately stored in sterile containers at -80 °C till further analysis by ELISA.
Results: The concentration of TNF-α was significantly higher in PAN (p≤0.0342), while there was no significant difference in TGF-β levels between the two groups (p≤0.7737). The levels of TNF-α were higher in subjects with OSMF in both the groups.
Conclusions: Pro- oncogenic cytokines like TNF-α are significantly raised in PAN and in those with OSMF even after cessation of the AN habit. Role of TNF-α inhibitors in prevention of oral carcinogenesis in both CAN and PAN needs to be investigated. Although habit abstinence is imperative for risk reduction of oral cancer in AN users, regular surveillance for malignant transformation is required in both current and past AN users.