IADR Abstract Archives
Silver Nanoparticle-Containing Resin-Based Dental Composite Materials for Sustainable Antimicrobial Activity
Objectives: A new approach has been developed to modify the surface of silver nanoparticles (AgNPs) with photoactivatable, methacrylate-containing molecules (C=C-modified AgNPs). The specialised surface modification enables the AgNPs to participate in light-activated setting reactions of methacrylate-based materials. The result is AgNP incorporation into resin-based dental materials (e.g., resin modified GICs, light-cured pit and fissure sealants, root canal sealers, resin blocks for CAD/CAM-fabrication) through the formation of strong covalent bonds throughout the resin matrix. This is thought to increase internal cross-linking density and prevent the migration of AgNPs out of the composite and into the surrounding environment as this would otherwise lead to a loss of antimicrobial efficacy. Thus, the objective of the study is the development of silver containing-resin-based composite materials offering sustained antibacterial/antibiofilm activity.
Methods: AgNPs functionalised with terminal methacrylate groups were synthesised using a microemulsion method, then characterised with Raman, infrared and nuclear magnetic resonance spectroscopy, transmission electron microscopy and dynamic light scattering. Compression and flexural strength testing of AgNP-containing materials was performed on a Universal Testing Machine following ISO 9917-1. Oral-associated bacterial biofilm accumulation was assessed using a viability stain with confocal laser scanning microscopy.
Results: Stable suspensions of <10 nm C=C-modified-AgNPs in ethanol were obtained and their successful incorporation into a variety of resin and resin-modified materials through photo-polymerisation reactions was demonstrated using a range of AgNP concentrations (i.e. tuneable loading was achieved). Resin-modified GICs containing AgNPs exhibited a strong antibiofilm effect against all bacteria tested, with no detectable silver leaching for at least one month (study ongoing).
Conclusions: C=C-modified AgNPs have shown significant promise to provide sustained, contact-based antimicrobial activity to a variety of resin and resin-modified dental materials, offering a new and versatile approach for managing and/or preventing oral infections.
Methods: AgNPs functionalised with terminal methacrylate groups were synthesised using a microemulsion method, then characterised with Raman, infrared and nuclear magnetic resonance spectroscopy, transmission electron microscopy and dynamic light scattering. Compression and flexural strength testing of AgNP-containing materials was performed on a Universal Testing Machine following ISO 9917-1. Oral-associated bacterial biofilm accumulation was assessed using a viability stain with confocal laser scanning microscopy.
Results: Stable suspensions of <10 nm C=C-modified-AgNPs in ethanol were obtained and their successful incorporation into a variety of resin and resin-modified materials through photo-polymerisation reactions was demonstrated using a range of AgNP concentrations (i.e. tuneable loading was achieved). Resin-modified GICs containing AgNPs exhibited a strong antibiofilm effect against all bacteria tested, with no detectable silver leaching for at least one month (study ongoing).
Conclusions: C=C-modified AgNPs have shown significant promise to provide sustained, contact-based antimicrobial activity to a variety of resin and resin-modified dental materials, offering a new and versatile approach for managing and/or preventing oral infections.