DENTURE PLAQUE and ASPIRATION PNEUMONIA, ANY RISK in OLDER PATIENT?

Objectives: To determine microbial profile of denture plaque,dental plaque and throat swab
To compare microbial profile of denture plaque, dental plaque, throat swab in dentate and denture wearer
To determine association of denture plaque microbial associaton with aspiration pneumonia.
Methods: Denture plaque, dental plaque and throat swab samples were collected from dentate and partial/complete edentulous older patient attending the Prosthodontic Clinic of the Restorative Dentistry Department, Lagos University Teaching Hospital, Lagos, Nigeria. Baseline samples and data from respiratory assessment were collected with final samples and respiratory data gathered about 2 months later. DNA extraction was conducted on all samples and also subjected to polymerase chain reaction (PCR). Bar-coded illumina MiSeq sequencing of bacterial hypervariable at V4 region of 16S rRNA was used to determine bacterial composition. Quantitative Insights into Microbial Ecology was used to process data sequencing, clustering in Operational Taxonomic Units (OTUs) and taxonomy was assigned.
Results: Bacterial diversity between denture plaque, dental plaque, throat swab compared and clinical signs of Aspiration Pneumonia monitored in 9 denture wearer (case group) and 9 dentate (control group) participants. Denture, dental plaque and throat swab were dominated with phyla Formicutes and pretobacteria. Major genus was Bacillus, Streptococcus and strenotrophonomas.
Denture plaque had a huge abundance of pretobacterium of the genus stenotrophonomas and denture wearer throat swab had none of the phylum proteobacterium and pseudomonas species, stenotrophonomas, when compared to the control (dental plaque), having relative abundance of the phylum proteobacterium and the genus stenotrophomonas and mattophilia. Similar phyla were maintained in line with denture plaque but one patient had abundant species of serattia (89.5%). The throat swabs were also dominated by the phyla proteobacteria, arcinetobacter, formicutes as the species pseudomonas and veronella in one control and the other by streptococcus, and some unknown specie (29.94%).
Conclusions: There is bacterial diversity in denture, dental plaque and throat swab. Bacterial diversity between denture wearers and dentate patients suggesting lesser risk of aspiration pneumonia in older patient.