IADR Abstract Archives
Localization of Fusobacterium Nucleatum in Oral Squamous Cell Carcinoma and Its Possible Directly Interacting Protein Molecules
Objectives: While 15% to 20% of cancers are associated with microbial infection, the relationship between oral microorganisms and oral squamous cell carcinoma (OSCC) remains unclear. In this study, 16S rDNA method was used to analyze the bacterial diversity in clinical OSCC tissue samples and tumor distant normal tissues, and the results showed that the detected amount of the oral symbiotic bacteria Fusobacterium nucleatum in OSCC tumor tissues was significantly higher than that in tumor distant normal tissues.
Methods: The position of Fusobacterium nucleatum was analyzed by fluorescence in situ hybridization (FISH) and the expression of CK, CD31, CD45, CD68, Cyclin D1, β-catenin, E-cadherin, NF-κB, HIF-1α was analyzed by immunohistochemistry (IHC) in OSCC tumor tissues and tumor distant normal tissues.
Results: Fusobacterium nucleatum was enriched in the tumor stroma where CD31+ blood vessels and inflammatory cells (including CD45+ leukocytes and CD68+ macrophages) are densely distributed. Cyclin D1 was mainly expressed in the nucleus of tumor cells. β-catenin was expressed in tumor cell membrane and positive in tumor interstitial vascular endothelial cells. E-cadherin is mainly expressed in tumor cell membranes. NF-κB was positively expressed in cytoplasm of tumor cells, tumor interstitial cells and myo-fibrocytes. HIF-1α is mainly expressed in the cytoplasm of tumor interstitial cells. HIF-1α was highly expressed where Fusobacterium nucleatum was densely distributed.
Conclusions: According to our study, Fusobacterium nucleatum might promote inflammatory response and hypoxia by interacting with NF-κB and HIF-1α in Oral squamous cell carcinoma.
Methods: The position of Fusobacterium nucleatum was analyzed by fluorescence in situ hybridization (FISH) and the expression of CK, CD31, CD45, CD68, Cyclin D1, β-catenin, E-cadherin, NF-κB, HIF-1α was analyzed by immunohistochemistry (IHC) in OSCC tumor tissues and tumor distant normal tissues.
Results: Fusobacterium nucleatum was enriched in the tumor stroma where CD31+ blood vessels and inflammatory cells (including CD45+ leukocytes and CD68+ macrophages) are densely distributed. Cyclin D1 was mainly expressed in the nucleus of tumor cells. β-catenin was expressed in tumor cell membrane and positive in tumor interstitial vascular endothelial cells. E-cadherin is mainly expressed in tumor cell membranes. NF-κB was positively expressed in cytoplasm of tumor cells, tumor interstitial cells and myo-fibrocytes. HIF-1α is mainly expressed in the cytoplasm of tumor interstitial cells. HIF-1α was highly expressed where Fusobacterium nucleatum was densely distributed.
Conclusions: According to our study, Fusobacterium nucleatum might promote inflammatory response and hypoxia by interacting with NF-κB and HIF-1α in Oral squamous cell carcinoma.