IADR Abstract Archives
Brazilin Reverses the Osteogenesis Inhibition of HPDLSCs Under Inflammatory Microenvironment
Objectives: Inflammation control and periodontal tissue regeneration are the key issues in periodontitis treatment of hPDLSCs. Brazilin, marker components of Caesalpinia sappan, reportedly have anti-inflammatory properties. This study aimed to investigate the effects of brazilin on the anti-inflammatory and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) under inflammatory conditions.
Methods: The safe concentrations of Brazilin on hPDLSCs were determined by CCK-8 assay. 10 μg/mL P. gingivalis lipopolysaccharide (Pg-LPS) was used to simulate the inflammatory microenvironment of periodontitis. The effects of Brazilin on inflammatory mediator production were measured by RT-PCR and Western blotting. RT-PCR, alkaline phosphatase (ALP) activity assay and Alizarin Red S staining were used to evaluate the levels of key osteogenic markers and calcified nodules.
Results: Brazilin (2.5μM, 5μM, 10μM) significantly decreased the production of IL-8, MMP1, and MMP3. Brazilin can promote the mRNA levels of classical osteogenic markers, including RUNX2, COL I, OCN, and OSX. Of note, it was found that 5 μM Brazilin had superior osteogenic promotion, ALP activity and Alizarin Red S staining, confirming this again. In addition, 5 μM Brazilin reversed the osteogenesis and proliferation inhibition of hPDLSCs attributed to Pg-LPS.
Conclusions: The effect of Brazilin presented in this study will expand the knowledge of its role in anti-inflammatory and osteogenic differentiation. Brazilin combined with hPDLSCs is expected to play an important role in the treatment of periodontal tissue regeneration.
Methods: The safe concentrations of Brazilin on hPDLSCs were determined by CCK-8 assay. 10 μg/mL P. gingivalis lipopolysaccharide (Pg-LPS) was used to simulate the inflammatory microenvironment of periodontitis. The effects of Brazilin on inflammatory mediator production were measured by RT-PCR and Western blotting. RT-PCR, alkaline phosphatase (ALP) activity assay and Alizarin Red S staining were used to evaluate the levels of key osteogenic markers and calcified nodules.
Results: Brazilin (2.5μM, 5μM, 10μM) significantly decreased the production of IL-8, MMP1, and MMP3. Brazilin can promote the mRNA levels of classical osteogenic markers, including RUNX2, COL I, OCN, and OSX. Of note, it was found that 5 μM Brazilin had superior osteogenic promotion, ALP activity and Alizarin Red S staining, confirming this again. In addition, 5 μM Brazilin reversed the osteogenesis and proliferation inhibition of hPDLSCs attributed to Pg-LPS.
Conclusions: The effect of Brazilin presented in this study will expand the knowledge of its role in anti-inflammatory and osteogenic differentiation. Brazilin combined with hPDLSCs is expected to play an important role in the treatment of periodontal tissue regeneration.