IADR Abstract Archives
Periodontitis-Oriented 16S RRNA Microbiome Profiles in the First Trimester Pregnancy
Objectives: To evaluate the microbiome profiles of subgingival plaque, saliva and stool of pregnant women during their first trimester.
Methods: A total of 54 Chinese mothers-to-be (20-35 years) during 7-13 weeks of pregnancy were recruited at Shenzhen Maternity & Child Healthcare Hospital from August 2020 to February 2021. Full-mouth periodontal examination was performed, and subgingival plaque, saliva and stool samples were collected. Total genomic DNA was extracted from all samples, and the V4 regions of 16S rRNA were amplified and sequenced. The raw data were analyzed using DADA2 pipeline to generate ASV counts and taxonomy assignment was undertaken with SILVA database. Microbiome analysis was performed by MicrobiomeAnalyst.
Results: Fusobacterium nucleatum (15.8%), Rothia dentocariosa (7.8%), R. aeria (6.4%), Porphyromonas gingivalis (6.3%) and Actinomyces oris (4.4%) were the top abundant species in plaque, while Prevotella melaninogenica (28.6%) was the predominant one in saliva. In stool, Faecalibacterium prausnitzii, Bacterioides vulgatus and B. uniformis were the most abundant species. Both alpha- and beta diversity of microbiome in plaque, saliva and stool did not vary between periodontitis (PD) and non-periodontitis (NPD) subjects. Differential Abundance Analysis revealed that B. fragilis appeared significantly decreased in PD patients. LEfSe analysis showed that Coprococcus comes was enriched in PD group while Akkermansia genus was highly enriched in NPD group. Random Forests analysis indicated that C. comes was the highest ranked species in gut microbiome. In plaque samples, the periodontopathogens P. gingivalis, Tannerella forsythia and F. nucleatum were enriched in PD group. For saliva samples, P. gingivalis profile significantly differed between the PD and NPD groups.
Conclusions: The present study suggests that oral microbiome may dramatically shift in pregnant women with existing periodontitis during the first trimester, and C. comes could be a potential indicator species of gut microbiome for maternal assessment. Further studies are needed to clarify these issues.
Methods: A total of 54 Chinese mothers-to-be (20-35 years) during 7-13 weeks of pregnancy were recruited at Shenzhen Maternity & Child Healthcare Hospital from August 2020 to February 2021. Full-mouth periodontal examination was performed, and subgingival plaque, saliva and stool samples were collected. Total genomic DNA was extracted from all samples, and the V4 regions of 16S rRNA were amplified and sequenced. The raw data were analyzed using DADA2 pipeline to generate ASV counts and taxonomy assignment was undertaken with SILVA database. Microbiome analysis was performed by MicrobiomeAnalyst.
Results: Fusobacterium nucleatum (15.8%), Rothia dentocariosa (7.8%), R. aeria (6.4%), Porphyromonas gingivalis (6.3%) and Actinomyces oris (4.4%) were the top abundant species in plaque, while Prevotella melaninogenica (28.6%) was the predominant one in saliva. In stool, Faecalibacterium prausnitzii, Bacterioides vulgatus and B. uniformis were the most abundant species. Both alpha- and beta diversity of microbiome in plaque, saliva and stool did not vary between periodontitis (PD) and non-periodontitis (NPD) subjects. Differential Abundance Analysis revealed that B. fragilis appeared significantly decreased in PD patients. LEfSe analysis showed that Coprococcus comes was enriched in PD group while Akkermansia genus was highly enriched in NPD group. Random Forests analysis indicated that C. comes was the highest ranked species in gut microbiome. In plaque samples, the periodontopathogens P. gingivalis, Tannerella forsythia and F. nucleatum were enriched in PD group. For saliva samples, P. gingivalis profile significantly differed between the PD and NPD groups.
Conclusions: The present study suggests that oral microbiome may dramatically shift in pregnant women with existing periodontitis during the first trimester, and C. comes could be a potential indicator species of gut microbiome for maternal assessment. Further studies are needed to clarify these issues.