IADR Abstract Archives
Overexpression of LncRNA-KAT7 Inhibited the Expression of Lipopolysaccharide-Induced Inflammatory Cytokine IL-17 in Periodontal Fibroblasts
Objectives: To investigate the effect of overexpression of lncRNA-KAT7 on the expression of LPS-induced inflammatory cytokine interleukin-17(IL-17) in human periodontal ligament fibroblasts (hPDLFs).
Methods: 24 patients with chronic periodontitis were selected as subjects, and 17 healthy volunteers were selected as the control group. The clinical data of the experimental group and the control group were collected and the expression levels of lncRNA-KAT7 and IL-17 in the gingival tissues of the two groups were detected by QRT-PCR, and the correlation between lncRNA-KAT7 and IL-17 was analyzed. Secondly, periodontal fibroblasts were isolated and cultured, and the expressions of lncRNA-KAT7 and IL-17 in periodontal fibroblasts from different sources were detected. Finally, periodontal membrane fibroblasts were treated with LPS following lncRNA-KAT7 upregulated, and the expression of IL-17 was observed.
Results: 1)LncRNA-KAT7 showed low expression and IL-17 showed high expression in chronic periodontitis tissues, and the two were negatively correlated. 2)The expression of lncRNA-KAT7 and IL-17 in periodontal fibroblasts from different tissues without LPS treatment showed no significant difference compared with the control group. 3)Without LPS treatment, overexpression of lncRNA-KAT7 did not affect IL-17 expression, but after LPS treatment, up-regulation of lncRNA-KAT7 significantly inhibited the expression of IL-17.
Conclusions: Upregulation of lncRNA-KAT7 in periodontal membrane fibroblasts can inhibit the expression of LPS-induced IL-17, which can provide a reference for the treatment of periodontitis.
Methods: 24 patients with chronic periodontitis were selected as subjects, and 17 healthy volunteers were selected as the control group. The clinical data of the experimental group and the control group were collected and the expression levels of lncRNA-KAT7 and IL-17 in the gingival tissues of the two groups were detected by QRT-PCR, and the correlation between lncRNA-KAT7 and IL-17 was analyzed. Secondly, periodontal fibroblasts were isolated and cultured, and the expressions of lncRNA-KAT7 and IL-17 in periodontal fibroblasts from different sources were detected. Finally, periodontal membrane fibroblasts were treated with LPS following lncRNA-KAT7 upregulated, and the expression of IL-17 was observed.
Results: 1)LncRNA-KAT7 showed low expression and IL-17 showed high expression in chronic periodontitis tissues, and the two were negatively correlated. 2)The expression of lncRNA-KAT7 and IL-17 in periodontal fibroblasts from different tissues without LPS treatment showed no significant difference compared with the control group. 3)Without LPS treatment, overexpression of lncRNA-KAT7 did not affect IL-17 expression, but after LPS treatment, up-regulation of lncRNA-KAT7 significantly inhibited the expression of IL-17.
Conclusions: Upregulation of lncRNA-KAT7 in periodontal membrane fibroblasts can inhibit the expression of LPS-induced IL-17, which can provide a reference for the treatment of periodontitis.