IADR Abstract Archives
Multifunctional Selenium-Doped Carbon Quantum Dots for Periodontitis Treatment.
Objectives: The purpose of this study was to investigate the in vitro antioxidant, anti-inflammatory, and osteogenic promotion effects of selenium-doped carbon quantum dots (Se-CQDs).
Methods: Se-CQDs were synthesized via the hydrothermal treatment of L-selenocysteine and Characterized. Cytotoxicity of Se-CQDs was analyzed with a CCK-8 assay. The antioxidant effect was detected by DCF-DA fluorescence staining, cell proliferation, and SOD activity assay in the model of H2O2-induced MC3T3-E1 oxidative injury. The osteogenic property was analyzed by ALP activity assay and ALP staining, as well as multiple osteoblast differentiation markers, including Runx2, OPN, and OPN. The anti-inflammation function was analyzed by gene and protein levels of pro-inflammatory cytokines.
Results: Se-CQDs were easily synthesized via the hydrothermal treatment of L-selenocysteine. CCK-8 assay suggested that Se-CQDs had a low-toxic and excellent biocompatibility. DCF-DA fluorescence staining, cell proliferation, and SOD activity assay showed that Se-CQDs exhibit a promising potential in scavenging ROS in the model of H2O2-induced MC3T3-E1 cells. ALP activity assay and ALP staining, as well as multiple osteoblast differentiation markers, including Runx2, OPN, and OPN, were significantly increased demonstrating that Se-CQDs had a good osteogenic activity of MC3T3-E1 cells and could rescue H2O2-induced osteoblasts dysfunction. Treatment of Porphyromonas gingivalis lipopolysaccharide-induced RAW264.7 cells with Se-CQDs suppressed gene and protein levels of pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6, and iNOS.
Conclusions: Our studies demonstrated that Se-CQDs were excellent for antioxidant, anti-inflammatory, and osteogenic acceleration, which showed great potential as an option for the treatment of periodontitis.
Methods: Se-CQDs were synthesized via the hydrothermal treatment of L-selenocysteine and Characterized. Cytotoxicity of Se-CQDs was analyzed with a CCK-8 assay. The antioxidant effect was detected by DCF-DA fluorescence staining, cell proliferation, and SOD activity assay in the model of H2O2-induced MC3T3-E1 oxidative injury. The osteogenic property was analyzed by ALP activity assay and ALP staining, as well as multiple osteoblast differentiation markers, including Runx2, OPN, and OPN. The anti-inflammation function was analyzed by gene and protein levels of pro-inflammatory cytokines.
Results: Se-CQDs were easily synthesized via the hydrothermal treatment of L-selenocysteine. CCK-8 assay suggested that Se-CQDs had a low-toxic and excellent biocompatibility. DCF-DA fluorescence staining, cell proliferation, and SOD activity assay showed that Se-CQDs exhibit a promising potential in scavenging ROS in the model of H2O2-induced MC3T3-E1 cells. ALP activity assay and ALP staining, as well as multiple osteoblast differentiation markers, including Runx2, OPN, and OPN, were significantly increased demonstrating that Se-CQDs had a good osteogenic activity of MC3T3-E1 cells and could rescue H2O2-induced osteoblasts dysfunction. Treatment of Porphyromonas gingivalis lipopolysaccharide-induced RAW264.7 cells with Se-CQDs suppressed gene and protein levels of pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6, and iNOS.
Conclusions: Our studies demonstrated that Se-CQDs were excellent for antioxidant, anti-inflammatory, and osteogenic acceleration, which showed great potential as an option for the treatment of periodontitis.
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