IADR Abstract Archives
Periodontal Tissue, Bone Microarchitecture and Cognitive Function in APP/PS1 Mice
Objectives: To investigate the periodontal tissue and bone microarchitecture changes in aged Alzheimer's disease (AD) animal models.
Methods: Using 12-month-old female amyloid precursor protein/presenilin 1(APP/PS1) transgenic mouse and wild type female littermates, this study evaluated the bone microarchitecture of posterior maxillary alveolar bone and femurs by micro-computed tomography (micro-CT). Morris water maze (MWM), passive avoidance test (PAT) and open field test (OFT) were performed to assess cognitive function. Pathological changes of periodontal tissue and femur were observed by histological chemistry. All animal experimental protocols were approved by the ethical committee of Animal Care and Experimental Committee of Shanghai Jiao Tong University of Medicine.
Results: Recognition and spatial memory was significantly impaired in aged APP/PS1 mice. Markedly differences were observed for the structural parameters of the BV/TV, BMD, Tb.Th, Tb.N, and Tb.Sp between the two groups. Compared to age-matched wild type mice, APP/PS1 mice existed more obvious periodontal destruction and bone degeneration.
Conclusions: Compared with wild type controls, more periodontal tissue (particularly the alveolar bone) damages were found in AD model mice. It provides a new insight into causal relationship between periodontitis and AD.
Methods: Using 12-month-old female amyloid precursor protein/presenilin 1(APP/PS1) transgenic mouse and wild type female littermates, this study evaluated the bone microarchitecture of posterior maxillary alveolar bone and femurs by micro-computed tomography (micro-CT). Morris water maze (MWM), passive avoidance test (PAT) and open field test (OFT) were performed to assess cognitive function. Pathological changes of periodontal tissue and femur were observed by histological chemistry. All animal experimental protocols were approved by the ethical committee of Animal Care and Experimental Committee of Shanghai Jiao Tong University of Medicine.
Results: Recognition and spatial memory was significantly impaired in aged APP/PS1 mice. Markedly differences were observed for the structural parameters of the BV/TV, BMD, Tb.Th, Tb.N, and Tb.Sp between the two groups. Compared to age-matched wild type mice, APP/PS1 mice existed more obvious periodontal destruction and bone degeneration.
Conclusions: Compared with wild type controls, more periodontal tissue (particularly the alveolar bone) damages were found in AD model mice. It provides a new insight into causal relationship between periodontitis and AD.